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Title: Metabolic Innovations Underpinning the Origin and Diversification of the Diatom Chloroplast
Authors: Nonoyama, Tomomi
Kazamia, Elena
Nawaly, Hermanus
Gao, Xia
Tsuji, Yoshinori  kyouindb  KAKEN_id
Matsuda, Yusuke
Bowler, Chris
Tanaka, Tsuyoshi
Dorrell, Richard G.
Author's alias: 野々山, 智美
辻, 敬典
松田, 祐介
田中, 剛
Keywords: MMETSP
stramenopiles
biotechnology
secondary endosymbiosis
isoprenoids
plastid proteome
Issue Date: 30-Jul-2019
Publisher: MDPI AG
Journal title: Biomolecules
Volume: 9
Issue: 8
Thesis number: 322
Abstract: Of all the eukaryotic algal groups, diatoms make the most substantial contributions to photosynthesis in the contemporary ocean. Understanding the biological innovations that have occurred in the diatom chloroplast may provide us with explanations to the ecological success of this lineage and clues as to how best to exploit the biology of these organisms for biotechnology. In this paper, we use multi-species transcriptome datasets to compare chloroplast metabolism pathways in diatoms to other algal lineages. We identify possible diatom-specific innovations in chloroplast metabolism, including the completion of tocopherol synthesis via a chloroplast-targeted tocopherol cyclase, a complete chloroplast ornithine cycle, and chloroplast-targeted proteins involved in iron acquisition and CO2 concentration not shared between diatoms and their closest relatives in the stramenopiles. We additionally present a detailed investigation of the chloroplast metabolism of the oil-producing diatom Fistulifera solaris, which is of industrial interest for biofuel production. These include modified amino acid and pyruvate hub metabolism that might enhance acetyl-coA production for chloroplast lipid biosynthesis and the presence of a chloroplast-localised squalene synthesis pathway unknown in other diatoms. Our data provides valuable insights into the biological adaptations underpinning an ecologically critical lineage, and how chloroplast metabolism can change even at a species level in extant algae.
Rights: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/243353
DOI(Published Version): 10.3390/biom9080322
PubMed ID: 31366180
Appears in Collections:Journal Articles

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