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Title: Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis
Authors: Sakanaka, Mikiyasu
Hansen, Morten Ejby
Gotoh, Aina
Katoh, Toshihiko  kyouindb  KAKEN_id
Yoshida, Keisuke
Odamaki, Toshitaka
Yachi, Hiroyuki
Sugiyama, Yuta
Kurihara, Shin
Hirose, Junko
Urashima, Tadasu
Xiao, Jin-zhong
Kitaoka, Motomitsu
Fukiya, Satoru
Yokota, Atsushi
Lo Leggio, Leila
Abou Hachem, Maher
Katayama, Takane  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 阪中, 幹祥
後藤, 愛那
加藤, 紀彦
吉田, 圭佑
小田巻, 俊孝
谷内, 寛之
杉山, 友太
栗原, 新
廣瀬, 潤子
浦島, 匡
清水(肖), 金忠
北岡, 本光
吹谷, 智
横田, 篤
片山, 高嶺
Issue Date: 2-Aug-2019
Publisher: American Association for the Advancement of Science (AAAS)
Journal title: Science Advances
Volume: 5
Issue: 8
Thesis number: eaaw7696
Abstract: The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that human milk oligosaccharides (HMOs) in breast milk promote the formation of a bifidobacteria-rich microbiota in infant guts; however, the underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA and HMO consumption analyses, combined with deposited metagenome data mining, revealed that FL transporter-2 is primarily associated with the bifidobacteria-rich microbiota formation in breast-fed infant guts. Structural analyses of the solute-binding protein (SBP) of FL transporter-2 complexed with 2′-fucosyllactose and 3-fucosyllactose, together with phylogenetic analysis of SBP homologs of both FL transporters, highlight a unique adaptation strategy of Bifidobacterium to HMOs, in which the gain-of-function mutations enable FL transporter-2 to efficiently capture major fucosylated HMOs. Our results provide a molecular insight into HMO-mediated symbiosis and coevolution between bifidobacteria and humans.
Description: ビフィズス菌におけるヒトへの適応進化を発見 --母乳オリゴ糖トランスポーターの獲得形質がビフィズスフローラ形成を促す--. 京都大学プレスリリース. 2019-09-05.
Rights: © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
DOI(Published Version): 10.1126/sciadv.aaw7696
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