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j.phytochem.2016.11.009.pdf | 1.7 MB | Adobe PDF | 見る/開く |
タイトル: | Molecular cloning and characterization of a Perilla frutescens cytochrome P450 enzyme that catalyzes the later steps of perillaldehyde biosynthesis |
著者: | Fujiwara, Yumi Ito, Michiho https://orcid.org/0000-0001-9821-0628 (unconfirmed) |
著者名の別形: | 伊藤, 美千穂 |
キーワード: | Perilla frutescens Labiatae Molecular cloning Biosynthetic pathway Cytochrome P450 Perillaldehyde synthase Perillyl alcohol synthase Monoterpenoid |
発行日: | Feb-2017 |
出版者: | Elsevier BV |
誌名: | Phytochemistry |
巻: | 134 |
開始ページ: | 26 |
終了ページ: | 37 |
抄録: | Perilla produces the cyclohexanoid monoterpene perillaldehyde as a major constituent of an essential oil that is accumulated in its glandular trichomes. Perillaldehyde is a marker compound for quality control of soyo and has biological activities such as antibacterial, sedative, or vasodilatory effects. The predicted perillaldehyde formation involves the cyclization of geranyl diphosphate, hydroxylation, and oxidation, and cytochrome P450 plays a crucial role in perillaldehyde biosynthesis. In this study, a cytochrome P450-type enzyme with perillyl alcohol and perillaldehyde synthase activities was isolated by analyzing an expressed sequence tag library from several oil types of pure lines of perilla. A recombinant protein with a sequence that was highly specific for the type of perillaldehyde was expressed in Saccharomyces cerevisiae and evaluated by an in vitro enzymatic reaction. The recombinant protein catalyzed the hydroxylation and oxidation of limonene to perillyl alcohol and perillaldehyde. Cytochrome P450 limonene-7-hydroxylase cDNA from Perilla frutescens has been previously isolated. The cytochrome P450 isolated in this study shares 37% amino-acid identity with the previously isolated enzyme; however, it may have different characteristics. |
著作権等: | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ The full-text file will be made open to the public on 1 February 2019 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/244234 |
DOI(出版社版): | 10.1016/j.phytochem.2016.11.009 |
PubMed ID: | 27890582 |
出現コレクション: | 学術雑誌掲載論文等 |
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