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タイトル: | Characterization of α-humulene synthases responsible for the production of sesquiterpenes induced by methyl jasmonate in Aquilaria cell culture |
著者: | Kumeta, Yukie Ito, Michiho https://orcid.org/0000-0001-9821-0628 (unconfirmed) |
著者名の別形: | 伊藤, 美千穂 |
キーワード: | Aquilaria crassna Cell cultures Functional characterization Methyl jasmonate treatment Terpene α-humulene synthase |
発行日: | Jul-2016 |
出版者: | Springer Nature |
誌名: | Journal of Natural Medicines |
巻: | 70 |
号: | 3 |
開始ページ: | 452 |
終了ページ: | 459 |
抄録: | Abstract: The resinous portions of Aquilaria and Gyrinops plants are known as ‘agarwood’ and have a distinctive fragrance. To examine the biosynthesis of these fragrant compounds, we previously established cell cultures of Aquilariacrassna in which the production of three sesquiterpenes (α-guaiene, α-humulene, and δ-guaiene) could be induced by methyl jasmonate (MJ), and showed that cloned δ-guaiene synthase from MJ-treated cells is involved in the synthesis of these three compounds, although only very small amounts of α-humulene are produced. In the present study, cDNAs encoding α-humulene synthases were also isolated. Three putative sesquiterpene synthase clones (AcHS1–3) isolated from the MJ-treated cells had very similar amino acid sequences and shared 52 % identity with δ-guaiene synthases. The recombinant enzymes catalyzed the formation of α-humulene as a major product. Expression of transcripts of the α-humulene synthase and δ-guaiene synthase genes in cultured cells increased after treatment with MJ. These results revealed that these α-humulene and δ-guaiene synthases are involved in the synthesis of three sesquiterpenes induced by MJ treatment. |
著作権等: | This is a post-peer-review, pre-copyedit version of an article published in Journal of Natural Medicines. The final authenticated version is available online at: http://dx.doi.org/10.1007/s11418-016-0999-8. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/244282 |
DOI(出版社版): | 10.1007/s11418-016-0999-8 |
PubMed ID: | 27180085 |
出現コレクション: | 学術雑誌掲載論文等 |
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