このアイテムのアクセス数: 701
このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| 978-1-4939-9740-4_16.pdf | 75.86 kB | Adobe PDF | 見る/開く |
完全メタデータレコード
| DCフィールド | 値 | 言語 |
|---|---|---|
| dc.contributor.author | Yamano, Takashi | en |
| dc.contributor.author | Fukuzawa, Hideya | en |
| dc.contributor.alternative | 山野, 隆志 | ja |
| dc.contributor.alternative | 福澤, 秀哉 | ja |
| dc.date.accessioned | 2019-11-18T04:16:52Z | - |
| dc.date.available | 2019-11-18T04:16:52Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.isbn | 9781493997398 | - |
| dc.identifier.issn | 1064-3745 | - |
| dc.identifier.uri | http://hdl.handle.net/2433/244795 | - |
| dc.description | Yamano T., Fukuzawa H. (2020) Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal. In: Li S., Chang L., Teissie J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY | en |
| dc.description.abstract | The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions. | en |
| dc.format.mimetype | application/pdf | - |
| dc.language.iso | eng | - |
| dc.publisher | Springer US | en |
| dc.rights | This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-9740-4_16. | en |
| dc.rights | The full-text file will be made open to the public on 30 August 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. | en |
| dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
| dc.rights | This is not the published version. Please cite only the published version. | en |
| dc.subject | Chlamydomonas reinhardtii | en |
| dc.subject | Electroporation | en |
| dc.subject | Photosynthesis | en |
| dc.subject | Protist | en |
| dc.subject | Square electric pulse | en |
| dc.title | Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal | en |
| dc.type | journal article | - |
| dc.type.niitype | Journal Article | - |
| dc.identifier.jtitle | Methods in Molecular Biology | en |
| dc.identifier.volume | 2050 | - |
| dc.identifier.spage | 155 | - |
| dc.identifier.epage | 161 | - |
| dc.relation.doi | 10.1007/978-1-4939-9740-4_16 | - |
| dc.textversion | author | - |
| dc.address | Graduate School of Biostudies, Kyoto University | en |
| dc.address | Graduate School of Biostudies, Kyoto University | en |
| dc.identifier.pmid | 31468489 | - |
| dcterms.accessRights | open access | - |
| datacite.date.available | 2020-08-30 | - |
| datacite.awardNumber | 25120714 | - |
| datacite.awardNumber | 16K07399 | - |
| jpcoar.funderName | 日本学術振興会 | ja |
| jpcoar.funderName | 日本学術振興会 | ja |
| jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
| jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
| 出現コレクション: | 学術雑誌掲載論文等 | |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。