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dc.contributor.author | Hia, Fabian | en |
dc.contributor.author | Yang, Sheng Fan | en |
dc.contributor.author | Shichino, Yuichi | en |
dc.contributor.author | Yoshinaga, Masanori | en |
dc.contributor.author | Murakawa, Yasuhiro | en |
dc.contributor.author | Vandenbon, Alexis | en |
dc.contributor.author | Fukao, Akira | en |
dc.contributor.author | Fujiwara, Toshinobu | en |
dc.contributor.author | Landthaler, Markus | en |
dc.contributor.author | Natsume, Tohru | en |
dc.contributor.author | Adachi, Shungo | en |
dc.contributor.author | Iwasaki, Shintaro | en |
dc.contributor.author | Takeuchi, Osamu | en |
dc.contributor.alternative | ヒヤ, フェビエン | ja |
dc.contributor.alternative | ヤン, シェン ファン | ja |
dc.contributor.alternative | 七野, 悠一 | ja |
dc.contributor.alternative | 吉永, 正憲 | ja |
dc.contributor.alternative | 村川, 泰裕 | ja |
dc.contributor.alternative | 深尾, 亜喜良 | ja |
dc.contributor.alternative | 藤原, 俊伸 | ja |
dc.contributor.alternative | 夏目, 徹 | ja |
dc.contributor.alternative | 足達, 俊吾 | ja |
dc.contributor.alternative | 岩崎, 信太郎 | ja |
dc.contributor.alternative | 竹内, 理 | ja |
dc.date.accessioned | 2019-11-21T05:09:15Z | - |
dc.date.available | 2019-11-21T05:09:15Z | - |
dc.date.issued | 2019-11-05 | - |
dc.identifier.issn | 1469-221X | - |
dc.identifier.issn | 1469-3178 | - |
dc.identifier.uri | http://hdl.handle.net/2433/244816 | - |
dc.description | ヒト細胞のコドン(遺伝暗号)に隠された暗号を解明 --ヒトコドン最適化制御による治療戦略の開発へ--. 京都大学プレスリリース. 2019-09-18. | ja |
dc.description.abstract | Codon bias has been implicated as one of the major factors contributing to mRNA stability in several model organisms. However, the molecular mechanisms of codon bias on mRNA stability remain unclear in humans. Here, we show that human cells possess a mechanism to modulate RNA stability through a unique codon bias. Bioinformatics analysis showed that codons could be clustered into two distinct groups--codons with G or C at the third base position (GC3) and codons with either A or T at the third base position (AT3): the former stabilizing while the latter destabilizing mRNA. Quantification of codon bias showed that increased GC3‐content entails proportionately higher GC‐content. Through bioinformatics, ribosome profiling, and in vitro analysis, we show that decoupling the effects of codon bias reveals two modes of mRNA regulation, one GC3‐ and one GC‐content dependent. Employing an immunoprecipitation‐based strategy, we identify ILF2 and ILF3 as RNA‐binding proteins that differentially regulate global mRNA abundances based on codon bias. Our results demonstrate that codon bias is a two‐pronged system that governs mRNA abundance. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | EMBO Press | en |
dc.rights | This is the author’s version of the paper, which has been published in final form at https://doi.org/10.15252/embr.201948220 | en |
dc.rights | The full-text file will be made open to the public on 3 March 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject | codon bias | en |
dc.subject | codon optimality | en |
dc.subject | GC-content | en |
dc.subject | mRNA stability | en |
dc.subject | transla-tion efficiency | en |
dc.title | Codon bias confers stability to human mRNAs | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | EMBO reports | en |
dc.identifier.volume | 20 | - |
dc.identifier.issue | 11 | - |
dc.relation.doi | 10.15252/embr.201948220 | - |
dc.textversion | author | - |
dc.identifier.artnum | e48220 | - |
dc.address | Department of Medical Chemistry, Graduate School of Medicine, Kyoto University | en |
dc.address | Department of Medical Chemistry, Graduate School of Medicine, Kyoto University | en |
dc.address | RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research | en |
dc.address | Department of Medical Chemistry, Graduate School of Medicine, Kyoto University | en |
dc.address | Division of Genomic Technologies, RIKEN Center for Life Science Technologies・RIKEN Preventive Medicine and Diagnosis Innovation Program | en |
dc.address | Laboratory of Infection and Prevention, Institute for Frontier Life and Medical Sciences, Kyoto University | en |
dc.address | Laboratory of Biochemistry, Department of Pharmacy, Kindai University | en |
dc.address | Laboratory of Biochemistry, Department of Pharmacy, Kindai University | en |
dc.address | RNA Biology and Posttranscriptional Regulation, Max Delbrück Center for Molecular Medicine Berlin, Berlin Institute for Molecular Systems Biology・IRI Life Sciences, Institut für Biologie, Humboldt‐Universität zu Berlin | en |
dc.address | Molecular Profiling Research Center for Drug Discovery (molprof), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo | en |
dc.address | Molecular Profiling Research Center for Drug Discovery (molprof), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo | en |
dc.address | RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research・Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo | en |
dc.address | Department of Medical Chemistry, Graduate School of Medicine, Kyoto University | en |
dc.identifier.pmid | 31482640 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2019-09-18 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2020-03-03 | - |
datacite.awardNumber | 18H05278 | - |
datacite.awardNumber | 17H05679 | - |
datacite.awardNumber | 17H04998 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
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