ダウンロード数: 200
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
BioProtoc.1864.pdf | 5 MB | Adobe PDF | 見る/開く |
タイトル: | Indirect Immunofluorescence Assay in Chlamydomonas reinhardtii |
著者: | Yamano, Takashi https://orcid.org/0000-0001-6578-8095 (unconfirmed) Fukuzawa, Hideya https://orcid.org/0000-0003-0963-5662 (unconfirmed) |
著者名の別形: | 山野, 隆志 福澤, 秀哉 |
発行日: | 5-Jul-2016 |
出版者: | Bio-Protocol, LLC |
誌名: | bio-protocol |
巻: | 6 |
号: | 13 |
論文番号: | e1854 |
抄録: | Determining the protein localization is essential to elucidate its in vivo function. Fluorescence-tagged proteins are widely used for it, but it is sometimes difficult to express tagged proteins in Chlamydomonas. Alternatively, indirect immunofluorescence assay is also one of the widely used methods and many reports determining the localization of Chlamydomonas proteins using this method are published. Here, we introduce a protocol of indirect immunofluorescence assay adapted from our papers reporting LCIB (CO₂-recycling factor in the vicinity of pyrenoid; Yamano et al., 2010), LCI1 (plasma membrane-localized inorganic carbon transporter; Ohnishi et al., 2010), HLA3 (plasma membrane-localized ABC-type bicarbonate transporter; Yamano et al., 2015), and LCIA (chloroplast envelope anion channel; Yamano et al., 2015) in Chlamydomonas reinhardtii. The protocol described here could be useful for observing the protein of interest in other algae cells. |
著作権等: | © 2016 The Authors; exclusive licensee Bio-protocol LLC. 許諾条件に基づいて掲載しています。 |
URI: | http://hdl.handle.net/2433/244823 |
DOI(出版社版): | 10.21769/BioProtoc.1864 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。