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Title: Host selection of hematophagous leeches (Haemadipsa japonica): Implications for iDNA studies
Authors: Hanya, Goro  kyouindb  KAKEN_id  orcid (unconfirmed)
Morishima, Kaori
Koide, Tomoya
Otani, Yosuke
Hongo, Shun  kyouindb  KAKEN_id  orcid (unconfirmed)
Honda, Takeaki
Okamura, Hiroki
Higo, Yuma
Hattori, Masamichi
Kondo, Yuki
Kurihara, Yosuke
Jin, Sakura
Otake, Aji
Shiroisihi, Izumi
Takakuwa, Tomomi
Yamamoto, Hiroki
Suzuki, Hanami
Kajimura, Hisashi
Hayakawa, Takashi
Suzuki‐Hashido, Nami
Nakano, Takafumi  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 半谷, 吾郎
本郷, 峻
本田, 剛章
岡村, 弘樹
肥後, 悠馬
栗原, 洋介
中野, 隆文
Keywords: barcoding
Japanese macaque
sika deer
Issue Date: Nov-2019
Publisher: Wiley
Journal title: Ecological Research
Volume: 34
Issue: 6
Start page: 842
End page: 855
Abstract: The development of an efficient and cost‐effective method for monitoring animal populations or biodiversity is urgently needed, and invertebrate‐derived DNA (iDNA) may offer a promising tool for assessing the diversity and other ecological information of vertebrates. We studied the host species of a hematophagous leech (Haemadipsa japonica) in Yakushima by genetic barcoding and compared the results with those for mammal composition revealed by camera trapping. We analyzed 119 samples using two sets of primers by Sanger sequencing and one set of primer by next generation sequencing. The proportion of the samples that were successfully sequenced and identified to at least one species was 11.8–24.3%, depending on the three different methods. In all of these three methods, most of the samples were identified as sika deer (18/20, 6/15 and 16/29) or human (2/20, 7/15 and 21/29). The nonhuman mammal host species composition was significantly different from that estimated by camera trapping. Sika deer was the main host, which may be related with their high abundance, large body size and terrestriality. Ten samples included DNA derived from multiple species of vertebrates. This may be due to the contamination of human DNA, but we also found DNA from deer, Japanese macaque and a frog in the same samples, suggesting the mixture of the two meals in the gut of the leech. Using H. japonica‐derived iDNA would not be suitable to make an inventory of species, but it may be useful to collect genetic information on the targeted species, due to their high host selectivity.
Rights: This is the peer reviewed version of the following article: [Ecological Research, 34(6), 842-855], which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
The full-text file will be made open to the public on 21 November 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1111/1440-1703.12059
Appears in Collections:Journal Articles

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