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dc.contributor.author | Thumkeo, D. | en |
dc.contributor.author | Katsura, Y. | en |
dc.contributor.author | Nishimura, Y. | en |
dc.contributor.author | Kanchanawong, P. | en |
dc.contributor.author | Tohyama, K. | en |
dc.contributor.author | Ishizaki, T. | en |
dc.contributor.author | Kitajima, S. | en |
dc.contributor.author | Takahashi, C. | en |
dc.contributor.author | Hirata, T. | en |
dc.contributor.author | Watanabe, N. | en |
dc.contributor.author | Krummel, M. F. | en |
dc.contributor.author | Narumiya, S. | en |
dc.contributor.alternative | タムケオ, ディーン | ja |
dc.contributor.alternative | 桂, 義親 | ja |
dc.contributor.alternative | 西村, 有香子 | ja |
dc.contributor.alternative | 通山, 潔 | ja |
dc.contributor.alternative | 石崎, 敏理 | ja |
dc.contributor.alternative | 北嶋, 俊輔 | ja |
dc.contributor.alternative | 高橋, 智聡 | ja |
dc.contributor.alternative | 平田, 多佳子 | ja |
dc.contributor.alternative | 渡邊, 直樹 | ja |
dc.contributor.alternative | 成宮, 周 | ja |
dc.date.accessioned | 2020-01-06T05:26:02Z | - |
dc.date.available | 2020-01-06T05:26:02Z | - |
dc.date.issued | 2020-01-01 | - |
dc.identifier.issn | 2375-2548 | - |
dc.identifier.uri | http://hdl.handle.net/2433/245246 | - |
dc.description | T細胞受容体シグナル伝達を細胞骨格アクチンが促進する機能を解明 --感染防御・自己免疫疾患・白血病の中心的細胞の活性化メカニズム解析--. 京都大学プレスリリース. 2020-01-06. | ja |
dc.description.abstract | The mechanism by which the cytosolic protein Zap70 physically interacts with and phosphorylates its substrate, the transmembrane protein LAT, upon T cell receptor (TCR) stimulation remains largely obscure. In this study, we found that the pharmacological inhibition of formins, a major class of actin nucleators, suppressed LAT phosphorylation by Zap70, despite TCR stimulation–dependent phosphorylation of Zap70 remaining intact. High-resolution imaging and three-dimensional image reconstruction revealed that localization of phosphorylated Zap70 to the immune synapse (IS) and subsequent LAT phosphorylation are critically dependent on formin-mediated actin polymerization. Using knockout mice, we identify mDia1 and mDia3, which are highly expressed in T cells and which localize to the IS upon TCR activation, as the critical formins mediating this process. Our findings therefore describe previously unsuspected roles for mDia1 and mDia3 in the spatiotemporal control of Zap70-dependent LAT phosphorylation at the IS through regulation of filamentous actin, and underscore their physiological importance in TCR signaling. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Association for the Advancement of Science (AAAS) | en |
dc.rights | © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. | en |
dc.title | mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by Zap70 at the immune synapse | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Science Advances | en |
dc.identifier.volume | 6 | - |
dc.identifier.issue | 1 | - |
dc.relation.doi | 10.1126/sciadv.aay2432 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | eaay2432 | - |
dc.identifier.pmid | 31911947 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2020-01-06-0 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 25860188 | - |
datacite.awardNumber | 15K18986 | - |
datacite.awardNumber | 19H01020 | - |
datacite.awardNumber | 17H01531 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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