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タイトル: | Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function |
著者: | Momotori, Naoki Jo, Jun ichiro Tabata, Yasuhiko |
著者名の別形: | 城, 潤一郎 田畑, 泰彦 |
キーワード: | Macrophages Pioglitazone Drug delivery system Poly(L-lactic-co-glycolic acid) PLGA Microspheres |
発行日: | 1-Dec-2019 |
出版者: | Elsevier BV |
誌名: | Regenerative Therapy |
巻: | 11 |
開始ページ: | 131 |
終了ページ: | 138 |
抄録: | Introduction: Macrophages play an important role in regulating inflammation and tissue regeneration. It is known that anti-inflammatory macrophages play an important role for tissue regeneration. The objective of this study is to modify macrophages phenotypes for anti-inflammatory function by utilizing drug delivery technology. Method: In this study, 4 types of poly (L-lactic-co-glycolic acid) (PLGA) microspheres incorporating pioglitazone of an anti-inflammatory modifier (pio-MS) with different sizes were prepared. In vitro release test of pio-MS was performed in phosphate buffered-saline solution (PBS) containing 1 wt% of sodium lauryl sulfate. The arginase activity and the secretion of interleukin (IL)−10 as anti-inflammatory macrophage markers of mouse bone marrow derived-macrophages (BMDM) cultured with the pio-MS were evaluated. Results: The sustained release of pioglitazone was observed from all types of pio-MS in vitro. When BMDM were cultured with the pio-MS with an average diameter of 40 μm (pio-MS40), the arginase activity and the secretion of IL-10 increased to a significant extent compared with other pio-MS. Conclusions: The pio-MS40 with an diameter of 40 μm had a potential to induce the anti-inflammatory modification of BMDM in this culture system. The sustained release of pioglitazone is promoting to modify the macrophage function. |
著作権等: | © 2019, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/245559 |
DOI(出版社版): | 10.1016/j.reth.2019.06.008 |
PubMed ID: | 31338392 |
出現コレクション: | 学術雑誌掲載論文等 |
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