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dc.contributor.author | Shimoda, Asako | en |
dc.contributor.author | Sawada, Shin ichi | en |
dc.contributor.author | Sasaki, Yoshihiro | en |
dc.contributor.author | Akiyoshi, Kazunari | en |
dc.contributor.alternative | 下田, 麻子 | ja |
dc.contributor.alternative | 澤田, 晋一 | ja |
dc.contributor.alternative | 佐々木, 善浩 | ja |
dc.contributor.alternative | 秋吉, 一成 | ja |
dc.date.accessioned | 2020-02-14T06:02:28Z | - |
dc.date.available | 2020-02-14T06:02:28Z | - |
dc.date.issued | 2019-08-08 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2433/245671 | - |
dc.description.abstract | Extracellular vesicles (EVs) carry information between cells in the form of biomolecules. Such molecules have been found to serve as biomarkers. Glycans attached to surface molecules on EVs are involved in their cellular uptake. In this study, we examined glycan profiles of small EVs which are generally termed exosomes before and after osteogenic differentiation of adipose-derived mesenchymal stem cells (MSCs) by an evanescent field fluorescence-assisted (EFF)-lectin array system to discover glycan biomarkers for osteogenic differentiation. We found few differences between exosomes before and after osteogenic differentiation of MSCs in terms of fundamental characteristics such as size, morphology, and exosomal marker proteins. However, specific lectins bound strongly to exosomes from differentiated cells. Exosomes from osteogenically differentiated MSCs bound strongly to fucose- and mannose-binding lectins, especially at a high concentration of exosomes. In summary, we found that several lectins bound to exosomes from differentiated MSCs more strongly than to those from undifferentiated cells using an EFF-lectin array system, indicating that monitoring exosomal surface glycans may identify predictive indexes of osteogenic differentiation. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.title | Exosome surface glycans reflect osteogenic differentiation of mesenchymal stem cells: Profiling by an evanescent field fluorescence-assisted lectin array system | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Scientific Reports | en |
dc.identifier.volume | 9 | - |
dc.relation.doi | 10.1038/s41598-019-47760-x | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 11497 | - |
dc.address | Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University | en |
dc.address | Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University | en |
dc.address | Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University | en |
dc.address | Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University | en |
dc.identifier.pmid | 31395910 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 17K13029 | - |
dc.identifier.eissn | 2045-2322 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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