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s41598-019-48911-w.pdf | 2.51 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Inoue, Rintaro | en |
dc.contributor.author | Nakagawa, Tatsuo | en |
dc.contributor.author | Morishima, Ken | en |
dc.contributor.author | Sato, Nobuhiro | en |
dc.contributor.author | Okuda, Aya | en |
dc.contributor.author | Urade, Reiko | en |
dc.contributor.author | Yogo, Rina | en |
dc.contributor.author | Yanaka, Saeko | en |
dc.contributor.author | Yagi-Utsumi, Maho | en |
dc.contributor.author | Kato, Koichi | en |
dc.contributor.author | Omoto, Kazuki | en |
dc.contributor.author | Ito, Kazuki | en |
dc.contributor.author | Sugiyama, Masaaki | en |
dc.contributor.alternative | 井上, 倫太郎 | ja |
dc.contributor.alternative | 守島, 健 | ja |
dc.contributor.alternative | 佐藤, 信浩 | ja |
dc.contributor.alternative | 奥田, 綾 | ja |
dc.contributor.alternative | 裏出, 令子 | ja |
dc.contributor.alternative | 杉山, 正明 | ja |
dc.date.accessioned | 2020-02-25T06:26:32Z | - |
dc.date.available | 2020-02-25T06:26:32Z | - |
dc.date.issued | 2019-08-30 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2433/245805 | - |
dc.description.abstract | To understand a biological system, it is important to observe structures of biomolecules in the solution where the system is functionalized. Small-Angle X-ray Scattering coupled with Size Exclusion Chromatography (SEC-SAXS) is one of techniques to selectively observe the target molecules in the multi-component system. However, this technique is believed to be available only with a synchrotron-based SAXS instrument due to requirement of high beam intensity and, therefore, the limitation of the beam time was obstacle to satisfy demands from many bio-researchers. We newly developed Laboratory-based Size exclusion chromatography SAXS System (La-SSS) by utilizing a latest laboratory-based SAXS instrument and finely optimization of the balance between flow rate, cell volume, irradiation time and so on. La-SSS succeeded not only decoupling of target protein(s) from non-specific aggregates but also measurement of each concerned component in a multi-component system. In addition, an option: “stopping mode”, which is designed for improving statistics of SAXS profile, realized a high S/N data acquisition for the most interesting protein in a multi-component system. Furthermore, by utilizing a column having small bed volume, the small-scale SEC-SAXS study makes available. Through optimization of instrumental parameters and environments, La-SSS is highly applicable for experimental requirements from various biological samples. It is strongly expected that a La-SSS concept must be a normal option for laboratory-based SAXS in the near future. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Science and Business Media LLC | en |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.title | Newly developed Laboratory-based Size exclusion chromatography Small-angle x-ray scattering System (La-SSS) | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Scientific Reports | en |
dc.identifier.volume | 9 | - |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 12 | - |
dc.relation.doi | 10.1038/s41598-019-48911-w | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 31471544 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | JP17K07361 | - |
datacite.awardNumber | JP19K16088 | - |
datacite.awardNumber | JP17K07816 | - |
datacite.awardNumber | JP18H05229 | - |
datacite.awardNumber | JP18H05534 | - |
datacite.awardNumber | JP18H03681 | - |
datacite.awardNumber | JP18K14892 | - |
datacite.awardNumber | JP18H05203 | - |
datacite.awardNumber | JP1901017 | - |
datacite.awardNumber | JP19J150602 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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