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dc.contributor.author | Parr, Callum J C | en |
dc.contributor.author | Wada, Shunsuke | en |
dc.contributor.author | Kotake, Kenjiro | en |
dc.contributor.author | Kameda, Shigetoshi | en |
dc.contributor.author | Matsuura, Satoshi | en |
dc.contributor.author | Sakashita, Souhei | en |
dc.contributor.author | Park, Soyoung | en |
dc.contributor.author | Sugiyama, Hiroshi | en |
dc.contributor.author | Kuang, Yi | en |
dc.contributor.author | Saito, Hirohide | en |
dc.contributor.alternative | 和田, 俊輔 | ja |
dc.contributor.alternative | 小武, 健二郎 | ja |
dc.contributor.alternative | 亀田, 重賢 | ja |
dc.contributor.alternative | 松浦, 理史 | ja |
dc.contributor.alternative | 坂下, 宗平 | ja |
dc.contributor.alternative | 朴, 昭映 | ja |
dc.contributor.alternative | 杉山, 弘 | ja |
dc.contributor.alternative | 齊藤, 博英 | ja |
dc.date.accessioned | 2020-03-08T23:54:41Z | - |
dc.date.available | 2020-03-08T23:54:41Z | - |
dc.date.issued | 2020-04-06 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/2433/245907 | - |
dc.description | mRNAスイッチの性能を大幅に向上させることのできる修飾塩基を発見. 京都大学プレスリリース. 2020-03-06. | ja |
dc.description.abstract | Synthetic messenger RNA (mRNA) tools often use pseudouridine and 5-methyl cytidine as substitutions for uridine and cytidine to avoid the immune response and cytotoxicity induced by introducing mRNA into cells. However, the influence of base modifications on the functionality of the RNA tools is poorly understood. Here we show that synthetic mRNA switches containing N1-methylpseudouridine (m1Ψ) as a substitution of uridine substantially out-performed all other modified bases studied, exhibiting enhanced microRNA and protein sensitivity, better cell-type separation ability, and comparably low immune stimulation. We found that the observed phenomena stem from the high protein expression from m1Ψ containing mRNA and efficient translational repression in the presence of target microRNAs or proteins. In addition, synthetic gene circuits with m1Ψ significantly improve performance in cells. These findings indicate that synthetic mRNAs with m1Ψ modification have enormous potentials in the research and application of biofunctional RNA tools. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press (OUP) | en |
dc.rights | © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.title | N1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Nucleic Acids Research | en |
dc.identifier.volume | 48 | - |
dc.identifier.issue | 6 | - |
dc.relation.doi | 10.1093/nar/gkaa070 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e35 | - |
dc.identifier.pmid | 32090264 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2020-03-06-1 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 15H05722 | - |
datacite.awardNumber | 15H05722 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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