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dc.contributor.authorKonishi, Hidenorija
dc.contributor.authorHio, Mamoruja
dc.contributor.authorKobayashi, Masahiroja
dc.contributor.authorTakase, Ryuichija
dc.contributor.authorHashimoto, Wataruja
dc.contributor.alternative小西, 英仁ja
dc.contributor.alternative日尾, 守ja
dc.contributor.alternative小林, 将大ja
dc.contributor.alternative髙瀬, 隆一ja
dc.contributor.alternative橋本, 渉ja
dc.date.accessioned2020-03-09T00:25:56Z-
dc.date.available2020-03-09T00:25:56Z-
dc.date.issued2020-03-04ja
dc.identifier.issn2045-2322ja
dc.identifier.urihttp://hdl.handle.net/2433/245908-
dc.description植物多糖類を感知して接近する微生物の仕組みの一端を解明 --果皮廃棄物の有効活用へ期待--. 京都大学プレスリリース. 2020-03-06.ja
dc.description.abstractAs opposed to typical bacteria exhibiting chemotaxis towards low-molecular-weight substances, such as amino acids and mono/oligosaccharides, gram-negative Sphingomonas sp. strain A1 shows chemotaxis towards alginate and pectin polysaccharides. To identify the mechanism of chemotaxis towards macromolecules, a genomic fragment was isolated from the wild-type strain A1 through complementation with the mutant strain A1-M5 lacking chemotaxis towards pectin. This fragment contained several genes including sph1118. Through whole-genome sequencing of strain A1-M5, sph1118 was found to harbour a mutation. In fact, sph1118 disruptant lost chemotaxis towards pectin, and this deficiency was recovered by complementation with wild-type sph1118. Interestingly, the gene disruptant also exhibited decreased pectin assimilation. Furthermore, the gene product SPH1118 was expressed in recombinant E. coli cells, purified and characterised. Differential scanning fluorimetry and UV absorption spectroscopy revealed that SPH1118 specifically binds to pectin with a dissociation constant of 8.5 μM. Using binding assay and primary structure analysis, SPH1118 was predicted to be a periplasmic pectin-binding protein associated with an ATP-binding cassette transporter. This is the first report on the identification and characterisation of a protein triggering chemotaxis towards the macromolecule pectin as well as its assimilation.ja
dc.format.mimetypeapplication/pdf
dc.language.isoengja
dc.publisherSpringer Natureja
dc.rights© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.ja
dc.subjectBacteriologyja
dc.subjectPolysaccharidesja
dc.titleBacterial chemotaxis towards polysaccharide pectin by pectin-binding proteinja
dc.type.niitypeJournal Articleja
dc.identifier.jtitleScientific Reportsja
dc.identifier.volume10ja
dc.relation.doi10.1038/s41598-020-60274-1ja
dc.textversionpublisherja
dc.identifier.artnum3977ja
dc.identifier.pmid32132546ja
dc.relation.urlhttps://www.kyoto-u.ac.jp/ja/research-news/2020-03-06ja
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