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dc.contributor.author | Kijima, Takashi | en |
dc.contributor.author | Nakagawa, Hiroshi | en |
dc.contributor.author | Shimonosono, Masataka | en |
dc.contributor.author | Chandramouleeswaran, Prasanna M. | en |
dc.contributor.author | Hara, Takeo | en |
dc.contributor.author | Sahu, Varun | en |
dc.contributor.author | Kasagi, Yuta | en |
dc.contributor.author | Kikuchi, Osamu | en |
dc.contributor.author | Tanaka, Koji | en |
dc.contributor.author | Giroux, Veronique | en |
dc.contributor.author | Muir, Amanda B. | en |
dc.contributor.author | Whelan, Kelly A. | en |
dc.contributor.author | Ohashi, Shinya | en |
dc.contributor.author | Naganuma, Seiji | en |
dc.contributor.author | Klein-Szanto, Andres J. | en |
dc.contributor.author | Shinden, Yoshiaki | en |
dc.contributor.author | Sasaki, Ken | en |
dc.contributor.author | Omoto, Itaru | en |
dc.contributor.author | Kita, Yoshiaki | en |
dc.contributor.author | Muto, Manabu | en |
dc.contributor.author | Bass, Adam J. | en |
dc.contributor.author | Diehl, J. Alan | en |
dc.contributor.author | Ginsberg, Gregory G. | en |
dc.contributor.author | Doki, Yuichiro | en |
dc.contributor.author | Mori, Masaki | en |
dc.contributor.author | Uchikado, Yasuto | en |
dc.contributor.author | Arigami, Takaaki | en |
dc.contributor.author | Avadhani, Narayan G. | en |
dc.contributor.author | Basu, Devraj | en |
dc.contributor.author | Rustgi, Anil K. | en |
dc.contributor.author | Natsugoe, Shoji | en |
dc.contributor.alternative | 貴島, 孝 | ja |
dc.contributor.alternative | 下之薗, 将貴 | ja |
dc.contributor.alternative | 原, 豪男 | ja |
dc.contributor.alternative | 菊池, 理 | ja |
dc.contributor.alternative | 田中, 晃司 | ja |
dc.contributor.alternative | 大橋, 真也 | ja |
dc.contributor.alternative | 長沼, 誠二 | ja |
dc.contributor.alternative | 新田, 吉陽 | ja |
dc.contributor.alternative | 佐々木, 健 | ja |
dc.contributor.alternative | 尾本, 至 | ja |
dc.contributor.alternative | 喜多, 芳昭 | ja |
dc.contributor.alternative | 武藤, 学 | ja |
dc.contributor.alternative | 土岐, 祐一郎 | ja |
dc.contributor.alternative | 森, 正樹 | ja |
dc.contributor.alternative | 内門, 泰斗 | ja |
dc.contributor.alternative | 有上, 貴明 | ja |
dc.contributor.alternative | 夏越, 祥次 | ja |
dc.date.accessioned | 2020-03-26T02:29:52Z | - |
dc.date.available | 2020-03-26T02:29:52Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 2352-345X | - |
dc.identifier.uri | http://hdl.handle.net/2433/246431 | - |
dc.description.abstract | Background & Aims: Oropharyngeal and esophageal squamous cell carcinomas, especially the latter, are a lethal disease, featuring intratumoral cancer cell heterogeneity and therapy resistance. To facilitate cancer therapy in personalized medicine, three-dimensional (3D) organoids may be useful for functional characterization of cancer cells ex vivo. We investigated the feasibility and the utility of patient-derived 3D organoids of esophageal and oropharyngeal squamous cell carcinomas. Methods: We generated 3D organoids from paired biopsies representing tumors and adjacent normal mucosa from therapy-naïve patients and cell lines. We evaluated growth and structures of 3D organoids treated with 5-fluorouracil ex vivo. Results: Tumor-derived 3D organoids were grown successfully from 15 out of 21 patients (71.4%) and passaged with recapitulation of the histopathology of the original tumors. Successful formation of tumor-derived 3D organoids was associated significantly with poor response to presurgical neoadjuvant chemotherapy or chemoradiation therapy in informative patients (P = 0.0357, progressive and stable diseases, n = 10 vs. partial response, n = 6). The 3D organoid formation capability and 5-fluorouracil resistance were accounted for by cancer cells with high CD44 expression and autophagy, respectively. Such cancer cells were found to be enriched in patient-derived 3D organoids surviving 5-fluorouracil treatment. Conclusions: The single cell-based 3D organoid system may serve as a highly efficient platform to explore cancer therapeutics and therapy resistance mechanisms in conjunction with morphological and functional assays with implications for translation in personalized medicine. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2019 The Authors. Published by Elsevier Inc. on behalf of the AGA Institute. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en |
dc.subject | 3D Organoids | en |
dc.subject | Autophagy | en |
dc.subject | CD44 | en |
dc.subject | 5-Fluorouracil | en |
dc.title | Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Cellular and Molecular Gastroenterology and Hepatology | - |
dc.identifier.volume | 7 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 73 | - |
dc.identifier.epage | 91 | - |
dc.relation.doi | 10.1016/j.jcmgh.2018.09.003 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 30510992 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 17H04285 | - |
datacite.awardNumber | 15K10108 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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