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Title: A note on retrograde gene transfer efficiency and inflammatory response of lentiviral vectors pseudotyped with FuG-E vs. FuG-B2 glycoproteins
Authors: Tanabe, Soshi
Uezono, Shiori
Tsuge, Hitomi
Fujiwara, Maki
Miwa, Miki
Kato, Shigeki
Nakamura, Katsuki  kyouindb  KAKEN_id
Kobayashi, Kazuto
Inoue, Ken-ichi
Takada, Masahiko  kyouindb  KAKEN_id
Author's alias: 田辺, 創思
上園, 志織
柘植, 仁美
藤原, 真紀
三輪, 美樹
加藤, 成樹
中村, 克樹
小林, 和人
井上, 謙一
髙田, 昌彦
Keywords: Basal ganglia
Genetic vectors
Neural circuits
Targeted gene repair
Issue Date: 5-Mar-2019
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 9
Thesis number: 3567
Abstract: Pseudotyped lentiviral vectors give access to pathway-selective gene manipulation via retrograde transfer. Two types of such lentiviral vectors have been developed. One is the so-called NeuRet vector pseudotyped with fusion glycoprotein type E, which preferentially transduces neurons. The other is the so-called HiRet vector pseudotyped with fusion glycoprotein type B2, which permits gene transfer into both neurons and glial cells at the injection site. Although these vectors have been applied in many studies investigating neural network functions, it remains unclear which vector is more appropriate for retrograde gene delivery in the brain. To compare the gene transfer efficiency and inflammatory response of the NeuRet vs. HiRet vectors, each vector was injected into the striatum in macaque monkeys, common marmosets, and rats. It was revealed that retrograde gene delivery of the NeuRet vector was equal to or greater than that of the HiRet vector. Furthermore, inflammation characterized by microglial and lymphocytic infiltration occurred when the HiRet vector, but not the NeuRet vector, was injected into the primate brain. The present results indicate that the NeuRet vector is more suitable than the HiRet vector for retrograde gene transfer in the primate and rodent brains.
Rights: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/249994
DOI(Published Version): 10.1038/s41598-019-39535-1
PubMed ID: 30837514
Appears in Collections:Journal Articles

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