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Title: Genome-wide Identification of Tebufenozide Resistant Genes in the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae)
Authors: Uchibori-Asano, Miwa
Jouraku, Akiya
Uchiyama, Toru
Yokoi, Kakeru
Akiduki, Gaku
Suetsugu, Yoshitaka
Kobayashi, Tetsuya
Ozawa, Akihito
Minami, Saki
Ishizuka, Chiharu
Nakagawa, Yoshiaki  kyouindb  KAKEN_id
Daimon, Takaaki
Shinoda, Tetsuro
Author's alias: 浅野(内堀), 美和
上樂, 明也
内山, 徹
横井, 翔
秋月, 岳
末次, 克行
小林, 徹也
小澤, 朗人
中川, 好秋
大門, 高明
篠田, 徹郎
Keywords: Gene expression profiling
Genetic markers
Issue Date: 12-Mar-2019
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 9
Thesis number: 4203
Abstract: The smaller tea tortrix, Adoxophyes honmai, has developed strong resistance to tebufenozide, a diacylhydrazine-type (DAH) insecticide. Here, we investigated its mechanism by identifying genes responsible for the tebufenozide resistance using various next generation sequencing techniques. First, double-digest restriction site-associated DNA sequencing (ddRAD-seq) identified two candidate loci. Then, synteny analyses using A. honmai draft genome sequences revealed that one locus contained the ecdysone receptor gene (EcR) and the other multiple CYP9A subfamily P450 genes. RNA-seq and direct sequencing of EcR cDNAs found a single nucleotide polymorphism (SNP), which was tightly linked to tebufenozide resistance and generated an amino acid substitution in the ligand-binding domain. The binding affinity to tebufenozide was about 4 times lower in in vitro translated EcR of the resistant strain than in the susceptible strain. RNA-seq analyses identified commonly up-regulated genes in resistant strains, including CYP9A and choline/carboxylesterase (CCE) genes. RT-qPCR analysis and bioassays showed that the expression levels of several CYP9A and CCE genes were moderately correlated with tebufenozide resistance. Collectively, these results suggest that the reduced binding affinity of EcR is the main factor and the enhanced detoxification activity by some CYP9As and CCEs plays a supplementary role in tebufenozide resistance in A. honmai.
Rights: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/250000
DOI(Published Version): 10.1038/s41598-019-40863-5
PubMed ID: 30862839
Appears in Collections:Journal Articles

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