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Title: Algal protein kinase, Triacylglycerol Accumulation Regulator 1, modulates cell viability and gametogenesis in carbon/nitrogen imbalanced conditions
Authors: Shinkawa, Haruka
Kajikawa, Masataka
Nomura, Yuko
Ogura, Mayu
Sawaragi, Yuri
Yamano, Takashi  kyouindb  KAKEN_id  orcid (unconfirmed)
Nakagami, Hirofumi
Sugiyama, Naoyuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Ishihama, Yasushi  kyouindb  KAKEN_id  orcid (unconfirmed)
Kanesaki, Yu
Yoshikawa, Hirofumi
Fukuzawa, Hideya  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 新川, はるか
梶川, 昌孝
椹木, 裕里
山野, 隆志
杉山, 直幸
石濱, 泰
福澤, 秀哉
Issue Date: Apr-2019
Publisher: Oxford University Press (OUP)
Journal title: Plant Cell Physiology
Volume: 60
Issue: 4
Start page: 916
End page: 930
Abstract: Nutrient-deprived microalgae accumulate triacylglycerol (TAG) in lipid droplets. A dual-specificity tyrosine phosphorylation-regulated kinase, TAG accumulation regulator 1 (TAR1) has been shown to be required for acetate-dependent TAG accumulation and the degradation of chlorophyll and photosynthesis-related proteins in photomixotrophic nitrogen (N)-deficient conditions (Kajikawa et al. 2015). However, this previous report only examined particular condition. Here, we report that in photoautotrophic N-deficient conditions, tar1-1 cells, with a mutation in the TAR1 gene, maintained higher levels of cell viability and lower levels of hydrogen peroxide generation and accumulated higher levels of TAG and starch compared with those of wild-type (WT) cells with bubbling of air containing 5% carbon dioxide. Transcriptomic analyses suggested that genes involved in the scavenging of reactive oxygen species are not repressed in tar1-1 cells. In contrast, the mating efficiency and mRNA levels of key regulatory genes for gametogenesis, MID, MTD, and FUS, were suppressed in tar1-1 cells. Among the TAR1-dependent phosphopeptides deduced by phosphoproteomic analysis, protein kinases and enzymes related to N assimilation and carbon (C) metabolism are of particular interest. Characterization of these putative downstream factors may elucidate the molecular pathway whereby TAR1 mediates cellular propagation and C and N metabolism in C/N-imbalanced stress conditions.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in Plant Cell Physiology following peer review. The version of record Haruka Shinkawa, Masataka Kajikawa, Yuko Nomura, Mayu Ogura, Yuri Sawaragi, Takashi Yamano, Hirofumi Nakagami, Naoyuki Sugiyama, Yasushi Ishihama, Yu Kanesaki, Hirofumi Yoshikawa, Hideya Fukuzawa, Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions, Plant and Cell Physiology, Volume 60, Issue 4, April 2019, Pages 916–930 is available online at:
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1093/pcp/pcz010
PubMed ID: 30668822
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