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タイトル: | RNA-Seq reveals virus–virus and virus–plant interactions in nature |
著者: | Kamitani, Mari Nagano, Atsushi J. Honjo, Mie N. Kudoh, Hiroshi https://orcid.org/0000-0001-9777-4886 (unconfirmed) |
著者名の別形: | 神谷, 麻梨 永野, 惇 本庄, 三恵 工藤, 洋 |
キーワード: | Argonaute2 Arabidopsis halleri Arabidopsis halleri partitivirus 1 multiple infection RNA-Seq Turnip mosaic virus |
発行日: | Nov-2016 |
出版者: | Oxford University Press (OUP) |
誌名: | FEMS Microbiology Ecology |
巻: | 92 |
号: | 11 |
論文番号: | fiw176 |
抄録: | As research on plant viruses has focused mainly on crop diseases, little is known about these viruses in natural environments. To understand the ecology of viruses in natural systems, comprehensive information on virus–virus and virus–host interactions is required. We applied RNA-Seq to plants from a natural population of Arabidopsis halleri subsp. gemmifera to simultaneously determine the presence/absence of all sequence-reported viruses, identify novel viruses and quantify the host transcriptome. By introducing the criteria of read number and genome coverage, we detected infections by Turnip mosaic virus (TuMV), Cucumber mosaic virus and Brassica yellows virus. Active TuMV replication was observed by ultramicroscopy. De novo assembly further identified a novel partitivirus, Arabidopsis halleri partitivirus 1. Interestingly, virus reads reached a maximum level that was equivalent to that of the host's total mRNA, although asymptomatic infection was common. AhgAGO2, a key gene in host defence systems, was upregulated in TuMV-infected plants. Multiple infection was frequent in TuMV-infected leaves, suggesting that TuMV facilitates multiple infection, probably by suppressing host RNA silencing. Revealing hidden plant–virus interactions in nature can enhance our understanding of biological interactions and may have agricultural applications. |
著作権等: | © FEMS 2016. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
URI: | http://hdl.handle.net/2433/250508 |
DOI(出版社版): | 10.1093/femsec/fiw176 |
PubMed ID: | 27549115 |
出現コレクション: | 学術雑誌掲載論文等 |
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