ダウンロード数: 87
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
jcm8101546.pdf | 2.17 MB | Adobe PDF | 見る/開く |
タイトル: | A Dual Protective Effect of Intestinal Remote Ischemic Conditioning in a Rat Model of Total Hepatic Ischemia |
著者: | Czigany, Zoltan Hata, Koichiro https://orcid.org/0000-0002-3609-6396 (unconfirmed) Lai, Wei Schwandt, Timo Yamamoto, Yuzo Uemoto, Shinji Tolba, Rene H. |
著者名の別形: | 秦, 浩一郎 山本, 雄造 上本, 伸二 |
キーワード: | liver ischemia total hepatic ischemia ischemia reperfusion remote conditioning ischemic conditioning intestinal conditioning |
発行日: | Oct-2019 |
出版者: | MDPI AG |
誌名: | Journal of Clinical Medicine |
巻: | 8 |
号: | 10 |
論文番号: | 1546 |
抄録: | The present study aimed to investigate the effects of intestinal remote ischemic preconditioning (iRIC) on ischemia-reperfusion injury (IRI) and gut barrier integrity in a rat model of total hepatic ischemia (THI). Male Wistar rats (n = 50; 250–300 g) were randomly allocated into two experimental groups: RIC/Control. Thirty minutes of THI was induced by clamping the hepatoduodenal ligament. iRIC was applied as 4-min of ischemia followed by 11-min of reperfusion by clamping the superior mesenteric artery. Animals were sacrificed at 1, 2, 6, 24 h post-reperfusion (n = 5/group/timepoint). RIC of the gut significantly improved microcirculation of the ileum and the liver. Tissue ATP-levels were higher following iRIC (Liver: 1.34 ± 0.12 vs. 0.97 ± 0.20 μmol/g, p = 0.04) and hepatocellular injury was reduced significantly (ALT: 2409 ± 447 vs. 6613 ± 1117 IU/L, p = 0.003). Systemic- and portal venous IL-6 and TNF-alpha levels were markedly lower following iRIC, demonstrating a reduced inflammatory response. iRIC led to a structural and functional preservation of the intestinal barrier. These results suggest that iRIC might confer a potent protection against the detrimental effects of THI in rats via reducing IRI and systemic inflammatory responses and at the same time by mitigating the dramatic consequences of severe intestinal congestion and bacterial translocation. |
著作権等: | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
URI: | http://hdl.handle.net/2433/250763 |
DOI(出版社版): | 10.3390/jcm8101546 |
PubMed ID: | 31561505 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。