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タイトル: Effects of Tissue Pressure on Transgene Expression Characteristics via Renal Local Administration Routes from Ureter or Renal Artery in the Rat Kidney
著者: Oyama, Natsuko
Takahashi, Haruyuki
Kawaguchi, Maho
Miyamoto, Hirotaka
Nishida, Koyo
Tsurumaru, Masako
Nakashima, Mikiro
Yamashita, Fumiyoshi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3503-8696 (unconfirmed)
Hashida, Mitsuru
Kawakami, Shigeru
著者名の別形: 大山, 奈津子
川口, 真帆
宮元, 敬天
西田, 孝洋
鶴丸, 雅子
中嶋, 幹郎
山下, 富義
橋田, 充
川上, 茂
キーワード: naked pDNA
physical methods
pressure
gene transfection
kidney
local administration
renal artery
renal ureter
発行日: Feb-2020
出版者: MDPI AG
誌名: Pharmaceutics
巻: 12
号: 2
論文番号: 114
抄録: We previously developed a renal pressure-mediated transfection method (renal pressure method) as a kidney-specific in vivo gene delivery system. However, additional information on selecting other injection routes and applicable animals remains unclear. In this study, we selected renal arterial and ureteral injections as local administration routes and evaluated the characteristics of gene delivery such as efficacy, safety, and distribution in pressured kidney of rat. Immediately after the naked pDNA injection, via renal artery or ureter, the left kidney of the rat was pressured using a pressure controlling device. Transfection efficiency of the pressured kidney was about 100-fold higher than that of the injection only group in both administration routes. The optimal pressure intensity in the rat kidney was 1.2 N/cm2 for renal arterial injection and 0.9 N/cm2 for ureteral injection. We found that transgene expression site differs according to administration route: cortical fibroblasts and renal tubule in renal arterial injection and cortical and medullary tubule and medullary collecting duct in ureteral injection. This is the first report to demonstrate that the renal pressure method can also be effective, after renal arterial and ureteral injections, in rat kidney.
著作権等: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/250776
DOI(出版社版): 10.3390/pharmaceutics12020114
PubMed ID: 32024046
出現コレクション:学術雑誌掲載論文等

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