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Title: The effect of advanced glycation end products on cellular signaling molecules in skeletal muscle
Authors: Egawa, Tatsuro  kyouindb  KAKEN_id  orcid (unconfirmed)
Ohno, Yoshitaka
Yokoyama, Shingo
Goto, Ayumi
Ito, Rika
Hayashi, Tatsuya  kyouindb  KAKEN_id  orcid (unconfirmed)
Goto, Katsumasa
Author's alias: 江川, 達郎
Keywords: glycation
signal transducer and activator of transcription 3
extracellular signal-regulated kinase
reverse phase protein array
Issue Date: 25-Jul-2018
Publisher: The Japanese Society of Physical Fitness and Sports Medicine
Journal title: The Journal of Physical Fitness and Sports Medicine
Volume: 7
Issue: 4
Start page: 229
End page: 238
Abstract: The accumulation of advanced glycation end products (AGEs) in the body causes the pathogenesis of aging-related diseases by inhibiting the normal properties and functions of proteins and the modulation of cellular signal transduction. Glycation stress induced by AGEs accumulation has the potential to contribute to sarcopenia: age-related reductions in muscle mass, strength, and function. However, the molecular response to AGEs in skeletal muscle is not fully understood. Therefore, to understand changes in cellular signaling in response to AGEs, this study aimed to investigate the phosphorylation status of phosphoproteins in AGEs-treated skeletal muscle. Treatment of C2C12 skeletal muscle cells with glucose-induced AGEs (0.1 mg/mL) for 5 days suppressed myotube formation, and this was accompanied by Nε-carboxymethyl-lysine accumulation. Reverse phase protein array analysis revealed that treatment with AGEs (glyoxylic-, pyruvate-, glycolaldehyde-, and glucose-induced AGEs) increased phosphorylation at eight phosphorylation sites and decreased phosphorylation at 64 phosphorylation sites. The phosphorylation level of signal transducer and activator of transcription 3 (STAT3) Tyr⁷⁰⁵ was most enhanced, and that of extracellular signal-regulated kinase (ERK) Thr²⁰²/Tyr²⁰⁴ was most suppressed. Almost all phosphorylation sites related to insulin/insulin-like growth factor 1 signaling were downregulated by AGEs. Increased STAT3 Tyr⁷⁰⁵ phosphorylation and decreased ERK Thr²⁰²/Tyr²⁰⁴ phosphorylation were observed in the skeletal muscles of mice treated with a diet high in AGEs for 16 weeks. These findings suggest that AGE accumulation impairs cellular signal transduction pathways in skeletal muscle cells, and thereby has the potential to induce skeletal muscle loss.
Rights: JPFSM is published under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
DOI(Published Version): 10.7600/jpfsm.7.229
Appears in Collections:Journal Articles

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