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dc.contributor.author | Zhao, Mingming | en |
dc.contributor.author | Tazumi, Atsutoshi | en |
dc.contributor.author | Takayama, Satoru | en |
dc.contributor.author | Takenaka-Ninagawa, Nana | en |
dc.contributor.author | Nalbandian, Minas | en |
dc.contributor.author | Nagai, Miki | en |
dc.contributor.author | Nakamura, Yumi | en |
dc.contributor.author | Nakasa, Masanori | en |
dc.contributor.author | Watanabe, Akira | en |
dc.contributor.author | Ikeya, Makoto | en |
dc.contributor.author | Hotta, Akitsu | en |
dc.contributor.author | Ito, Yuta | en |
dc.contributor.author | Sato, Takahiko | en |
dc.contributor.author | Sakurai, Hidetoshi | en |
dc.contributor.alternative | 趙, 明明 | ja |
dc.contributor.alternative | 田積, 充年 | ja |
dc.contributor.alternative | 高山, 了 | ja |
dc.contributor.alternative | 竹中, 菜々 | ja |
dc.contributor.alternative | 中村, 友美 | ja |
dc.contributor.alternative | 中佐, 昌紀 | ja |
dc.contributor.alternative | 渡辺, 亮 | ja |
dc.contributor.alternative | 池谷, 真 | ja |
dc.contributor.alternative | 堀田, 秋津 | ja |
dc.contributor.alternative | 伊東, 佑太 | ja |
dc.contributor.alternative | 佐藤, 貴彦 | ja |
dc.contributor.alternative | 櫻井, 英俊 | ja |
dc.date.accessioned | 2020-07-21T00:46:10Z | - |
dc.date.available | 2020-07-21T00:46:10Z | - |
dc.date.issued | 2020-07-14 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2433/252790 | - |
dc.description | 筋ジストロフィーモデルマウスにおけるヒトiPS細胞由来骨格筋幹細胞の移植効果を確認. 京都大学プレスリリース. 2020-07-20. | ja |
dc.description.abstract | Duchenne muscular dystrophy (DMD) is a progressive and fatal muscle-wasting disease caused by DYSTROPHIN deficiency. Cell therapy using muscle stem cells (MuSCs) is a potential cure. Here, we report a differentiation method to generate fetal MuSCs from human induced pluripotent stem cells (iPSCs) by monitoring MYF5 expression. Gene expression profiling indicated that MYF5-positive cells in the late stage of differentiation have fetal MuSC characteristics, while MYF5-positive cells in the early stage of differentiation have early myogenic progenitor characteristics. Moreover, late-stage MYF5-positive cells demonstrated good muscle regeneration potential and produced DYSTROPHIN in vivo after transplantation into DMD model mice, resulting in muscle function recovery. The engrafted cells also generated PAX7-positive MuSC-like cells under the basal lamina of DYSTROPHIN-positive fibers. These findings suggest that MYF5-positive fetal MuSCs induced in the late stage of iPSC differentiation have cell therapy potential for DMD. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2020 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en |
dc.subject | muscular dystrophy | en |
dc.subject | pluripotent stem cells | en |
dc.subject | iPSC | en |
dc.subject | Pax7 | en |
dc.subject | Myf5 | en |
dc.subject | muslce stem cells | en |
dc.subject | myogenic differentiation | en |
dc.subject | WNT agonist | en |
dc.title | Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Stem cell report | en |
dc.identifier.volume | 15 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 80 | - |
dc.identifier.epage | 94 | - |
dc.relation.doi | 10.1016/j.stemcr.2020.06.004 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 32619494 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2020-07-20-1 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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