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dc.contributor.authorMukohira, Hisaen
dc.contributor.authorHara, Takahiroen
dc.contributor.authorAbe, Shinyaen
dc.contributor.authorTani-Ichi, Shizueen
dc.contributor.authorSehara-Fujisawa, Atsukoen
dc.contributor.authorNagasawa, Takashien
dc.contributor.authorTobe, Kazuyukien
dc.contributor.authorIkuta, Koichien
dc.contributor.alternative原, 崇裕ja
dc.contributor.alternative阿部, 真也ja
dc.contributor.alternative谷一, 靖江ja
dc.contributor.alternative生田, 宏一ja
dc.date.accessioned2020-08-03T05:14:38Z-
dc.date.available2020-08-03T05:14:38Z-
dc.date.issued2019-11-
dc.identifier.issn1460-2377-
dc.identifier.urihttp://hdl.handle.net/2433/253542-
dc.description.abstractStromal cells in bone marrow (BM) constitute a specific microenvironment supporting the development and maintenance of hematopoietic cells. Adiponectin is a cytokine secreted by adipocytes. Besides its anti-diabetic and anti-atherogenic roles, adiponectin reportedly regulates the development and function of hematopoietic cells in BM. However, it remains unclear whether mesenchymal stromal cells in BM express adiponectin. Here, we show that PDGFRβ+VCAM-1+ stromal cells express adiponectin. Lineage tracing revealed that a majority of PDGFRβ+VCAM-1+ cells were targeted by an adiponectin promoter-driven Cre (Adipoq-Cre) transgene. Additionally, the Adipoq-Cre transgene targets a minority of osteoblasts at a younger age but larger populations are targeted at an older age. Furthermore, the Adipoq-Cre transgene targets almost all CXCL12-abundant reticular (CAR) cells and most of the stromal cells targeted by the Adipoq-Cre transgene are CAR cells. Finally, deletion of interleukin-7 (IL-7) by the Adipoq-Cre transgene resulted in severe impairment of B lymphopoiesis in BM. These results demonstrate that PDGFRβ+VCAM-1+ stromal cells in BM express adiponectin and are targeted by the Adipoq-Cre transgene, suggesting a broader specificity of the Adipoq-Cre transgene.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherOxford University Press (OUP)en
dc.rightsThis is a pre-copyedited, author-produced version of an article accepted for publication in International immunology following peer review. The version of record Hisa Mukohira, Takahiro Hara, Shinya Abe, Shizue Tani-ichi, Atsuko Sehara-Fujisawa, Takashi Nagasawa, Kazuyuki Tobe, Koichi Ikuta, Mesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgene, International Immunology, Volume 31, Issue 11, November 2019, Pages 729–742 is available online at: https://doi.org/10.1093/intimm/dxz042.en
dc.rightsこの論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.rightsThis is not the published version. Please cite only the published version.en
dc.subjectadipocyteen
dc.subjectadiponectinen
dc.subjectbone marrowen
dc.subjectCXCL12en
dc.subjectIL-7en
dc.subjectstromal cellsen
dc.titleMesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgeneen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleInternational immunologyen
dc.identifier.volume31-
dc.identifier.issue11-
dc.identifier.spage729-
dc.identifier.epage742-
dc.relation.doi10.1093/intimm/dxz042-
dc.textversionauthor-
dc.addressLaboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto University・Graduate School of Medicine, Kyoto Universityen
dc.addressLaboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto Universityen
dc.addressLaboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto University・Graduate School of Medicine, Kyoto Universityen
dc.addressGraduate School of Medicine, Kyoto University・Graduate School of Medicine, Kyoto Universityen
dc.addressLaboratory of Tissue Stem Cell Biology, Department of Regeneration Science and Engineering, Institute of Frontier Life and Medical Sciences, Kyoto Universityen
dc.addressLaboratory of Stem Cell Biology and Developmental Immunology, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka Universityen
dc.addressFirst Department of Internal Medicine, Faculty of Medicine, University of Toyamaen
dc.addressLaboratory of Immune Regulation, Department of Virus Research, Institute of Frontier Life and Medical Sciences, Kyoto Universityen
dc.identifier.pmid31094421-
dcterms.accessRightsopen access-
datacite.awardNumber16K15288
datacite.awardNumber16H05172
datacite.awardNumber15H01153
datacite.awardNumber26460572
datacite.awardNumber16K08835
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
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