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タイトル: | Computer-assisted beat-pattern analysis and the flagellar waveforms of bovine spermatozoa |
著者: | Walker, Benjamin J. Phuyal, Shiva Ishimoto, Kenta Tung, Chih-Kuan Gaffney, Eamonn A. |
著者名の別形: | 石本, 健太 |
キーワード: | flagellar analysis spermatozoa computer-assisted sperm analysis |
発行日: | Jun-2020 |
出版者: | The Royal Society |
誌名: | Royal Society Open Science |
巻: | 7 |
号: | 6 |
論文番号: | 200769 |
抄録: | Obstructed by hurdles in information extraction, handling and processing, computer-assisted sperm analysis systems have typically not considered in detail the complex flagellar waveforms of spermatozoa, despite their defining role in cell motility. Recent developments in imaging techniques and data processing have produced significantly improved methods of waveform digitization. Here, we use these improvements to demonstrate that near-complete flagellar capture is realizable on the scale of hundreds of cells, and, further, that meaningful statistical comparisons of flagellar waveforms may be readily performed with widely available tools. Representing the advent of high-fidelity computer-assisted beat-pattern analysis, we show how such a statistical approach can distinguish between samples using complex flagellar beating patterns rather than crude summary statistics. Dimensionality-reduction techniques applied to entire samples also reveal qualitatively distinct components of the beat, and a novel data-driven methodology for the generation of representative synthetic waveform data is proposed. |
著作権等: | © 2020 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/254109 |
DOI(出版社版): | 10.1098/rsos.200769 |
PubMed ID: | 32742702 |
出現コレクション: | 学術雑誌掲載論文等 |
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