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dc.contributor.authorMatsumoto, Shomaen
dc.contributor.authorPorter, Christopher J.en
dc.contributor.authorOgasawara, Naomien
dc.contributor.authorIwatani, Chizuruen
dc.contributor.authorTsuchiya, Hideakien
dc.contributor.authorSeita, Yasunarien
dc.contributor.authorChang, Yu-Weien
dc.contributor.authorOkamoto, Ikuhiroen
dc.contributor.authorSaitou, Mitinorien
dc.contributor.authorEma, Masatsuguen
dc.contributor.authorPerkins, Theodore J.en
dc.contributor.authorStanford, William L.en
dc.contributor.authorTanaka, Satoshien
dc.contributor.alternative松本, 翔馬ja
dc.contributor.alternative岩谷, 千鶴ja
dc.contributor.alternative土屋, 英明ja
dc.contributor.alternative清田, 弥寿成ja
dc.contributor.alternative岡本, 郁弘ja
dc.contributor.alternative斎藤, 通紀ja
dc.contributor.alternative依馬, 正次ja
dc.contributor.alternative田中, 智ja
dc.date.accessioned2020-10-01T02:24:59Z-
dc.date.available2020-10-01T02:24:59Z-
dc.date.issued2020-04-22-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/2433/255223-
dc.description.abstractThe placenta forms a maternal-fetal junction that supports many physiological functions such as the supply of nutrition and exchange of gases and wastes. Establishing an in vitro culture model of human and non-human primate trophoblast stem/progenitor cells is important for investigating the process of early placental development and trophoblast differentiation. In this study, we have established five trophoblast stem cell (TSC) lines from cynomolgus monkey blastocysts, named macTSC #1-5. Fibroblast growth factor 4 (FGF4) enhanced proliferation of macTSCs, while other exogenous factors were not required to maintain their undifferentiated state. macTSCs showed a trophoblastic gene expression profile and trophoblast-like DNA methylation status and also exhibited differentiation capacity towards invasive trophoblast cells and multinucleated syncytia. In a xenogeneic chimera assay, these stem cells contributed to trophectoderm (TE) development in the chimeric blastocysts. macTSC are the first primate trophoblast cell lines whose proliferation is promoted by FGF4. These cell lines provide a valuable in vitro culture model to analyze the similarities and differences in placental development between human and non-human primates.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherSpringer Natureen
dc.rights© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectReproductive biologyen
dc.subjectStem cellsen
dc.titleEstablishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocystsen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleScientific Reportsen
dc.identifier.volume10-
dc.relation.doi10.1038/s41598-020-63602-7-
dc.textversionpublisher-
dc.identifier.artnum6827-
dc.identifier.pmid32321940-
dcterms.accessRightsopen access-
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