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j.stemcr.2019.05.029.pdf | 9.46 MB | Adobe PDF | 見る/開く |
タイトル: | Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids |
著者: | Sakaguchi, Hideya Ozaki, Yuki Ashida, Tomoka Matsubara, Takayoshi Oishi, Naotaka Kihara, Shunsuke Takahashi, Jun |
著者名の別形: | 坂口, 秀哉 尾崎, 由季 芦田, 知佳 松原, 孝宜 髙橋, 淳 |
キーワード: | cerebral organoid human pluripotent stem cell three-dimensional imaging calcium imaging neural network activity |
発行日: | 10-Sep-2019 |
出版者: | Elsevier BV |
誌名: | Stem Cell Reports |
巻: | 13 |
号: | 3 |
開始ページ: | 458 |
終了ページ: | 473 |
抄録: | The cerebrum is a major center for brain function, and its activity is derived from the assembly of activated cells in neural networks. It is currently difficult to study complex human cerebral neuronal network activity. Here, using cerebral organoids, we report self-organized and complex human neural network activities that include synchronized and non-synchronized patterns. Self-organized neuronal network formation was observed following a dissociation culture of human embryonic stem cell-derived cerebral organoids. The spontaneous individual and synchronized activity of the network was measured via calcium imaging, and subsequent analysis enabled the examination of detailed cell activity patterns, providing simultaneous raster plots, cluster analyses, and cell distribution data. Finally, we demonstrated the feasibility of our system to assess drug-inducible dynamic changes of the network activity. The comprehensive functional analysis of human neuronal networks using this system may offer a powerful tool to access human brain function. |
著作権等: | © 2019 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
URI: | http://hdl.handle.net/2433/255246 |
DOI(出版社版): | 10.1016/j.stemcr.2019.05.029 |
PubMed ID: | 31257131 |
出現コレクション: | 学術雑誌掲載論文等 |
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