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dc.contributor.author | Sugimoto, Misaki | en |
dc.contributor.author | Suda, Akiyo | en |
dc.contributor.author | Futaki, Shiroh | en |
dc.contributor.author | Imanishi, Miki | en |
dc.contributor.alternative | 須田, 明代 | ja |
dc.contributor.alternative | 二木, 史朗 | ja |
dc.contributor.alternative | 今西, 未来 | ja |
dc.date.accessioned | 2020-10-07T23:50:45Z | - |
dc.date.available | 2020-10-07T23:50:45Z | - |
dc.date.issued | 2020-10-01 | - |
dc.identifier.issn | 2076-3417 | - |
dc.identifier.uri | http://hdl.handle.net/2433/255382 | - |
dc.description.abstract | RNAs play important roles in gene expression through translation and RNA splicing. Regulation of specific RNAs is useful to understand and manipulate specific transcripts. Pumilio and fem-3 mRNA-binding factor (PUF) proteins, programmable RNA-binding proteins, are promising tools for regulating specific RNAs by fusing them with various functional domains. The key question is: How can PUF-based molecular tools efficiently regulate RNA functions? Here, we show that the combination of multiple PUF proteins, compared to using a single PUF protein, targeting independent RNA sequences at the 3′ untranslated region (UTR) of a target transcript caused cooperative effects to regulate the function of the target RNA by luciferase reporter assays. It is worth noting that a higher efficacy was achieved with smaller amounts of each PUF expression vector introduced into the cells compared to using a single PUF protein. This strategy not only efficiently regulates target RNA functions but would also be effective in reducing off-target effects due to the low doses of each expression vector. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | jpn | - |
dc.publisher | MDPI AG | en |
dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en |
dc.subject | RNA binding protein | en |
dc.subject | PUF | en |
dc.subject | RNA regulation | en |
dc.title | Effective RNA Regulation by Combination of Multiple Programmable RNA-Binding Proteins | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Applied Sciences | - |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 19 | - |
dc.relation.doi | 10.3390/app10196803 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 6803 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 19H02850 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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