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タイトル: | Kinetic analysis of inhibition of α-glucosidase by leaf powder from Morus australis and its component iminosugars |
著者: | Qiao, Ying Nakayama, Juri Ikeuchi, Takeaki Ito, Masaaki Kimura, Toshiyuki Kojima, Kenji Takita, Teisuke Yasukawa, Kiyoshi |
著者名の別形: | 池内, 健晃 兒島, 憲二 滝田, 禎亮 保川, 清 |
キーワード: | α-glucosidase fagomine iminosugar 1-DNJ Morus australis |
発行日: | 2-Oct-2020 |
出版者: | Informa UK Limited |
誌名: | Bioscience, Biotechnology, and Biochemistry |
巻: | 84 |
号: | 10 |
開始ページ: | 2149 |
終了ページ: | 2156 |
抄録: | Mulberry leaves contain iminosugars, such as 1-deoxynojirimycin (1-DNJ), fagomine, and 2-O-α-D-galactopyranosyl deoxynojirimycin (GAL-DNJ) that inhibit α-glucosidase. In this study, we quantified iminosugars in Morus australis leaves and made the kinetic analysis in the hydrolysis of maltose by α-glucosidase. By LC-MS/MS, the concentrations of 1-DNJ, fagomine, and GAL-DNJ in the powdered leaves were 4.0, 0.46, and 2.5 mg/g, respectively, and those in the roasted ones were 1.0, 0.24, and 0.73 mg/g, respectively, suggesting that the roasting process degraded iminosugars. Steady-state kinetic analysis revealed that the powdered and roasted leaves exhibited competitive inhibition. At pH 6.0 at 37ºC, the IC50 values of the extracts from the boiled powdered or roasted leaves were 0.36 and 1.1 mg/mL, respectively. At the same condition, the IC50 values of 1-DNJ, fagomine, and GAL-DNJ were 0.70 μg/mL, 0.18 mg/mL, and 2.9 mg/mL, respectively. These results suggested that in M. australis, 1-DNJ is a major inhibitor of α-glucosidase. |
著作権等: | This is an Accepted Manuscript of an article published by Taylor & Francis Group in 'Bioscience, Biotechnology, and Biochemistry' on 2020, available online at: http://www.tandfonline.com/10.1080/09168451.2020.1783991 The full-text file will be made open to the public on 13 Jul 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving' この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/255582 |
DOI(出版社版): | 10.1080/09168451.2020.1783991 |
PubMed ID: | 32660357 |
出現コレクション: | 学術雑誌掲載論文等 |
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