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ファイル | 記述 | サイズ | フォーマット | |
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eLife.61960.pdf | 2.67 MB | Adobe PDF | 見る/開く |
タイトル: | Single-cell dynamics of pannexin-1-facilitated programmed ATP loss during apoptosis |
著者: | Imamura, Hiromi https://orcid.org/0000-0002-1896-0443 (unconfirmed) Sakamoto, Shuichiro Yoshida, Tomoki Matsui, Yusuke Penuela, Silvia Laird, Dale W Mizukami, Shin Kikuchi, Kazuya Kakizuka, Akira |
著者名の別形: | 今村, 博臣 吉田, 有希 松井, 勇輔 水上, 進 菊地, 和也 垣塚, 彰 |
発行日: | 14-Oct-2020 |
出版者: | eLife Sciences Publications, Ltd |
誌名: | eLife |
巻: | 9 |
論文番号: | e61960 |
抄録: | ATP is essential for all living cells. However, how dead cells lose ATP has not been well investigated. In this study, we developed new FRET biosensors for dual imaging of intracellular ATP level and caspase-3 activity in single apoptotic cultured human cells. We show that the cytosolic ATP level starts to decrease immediately after the activation of caspase-3, and this process is completed typically within 2 hr. The ATP decrease was facilitated by caspase-dependent cleavage of the plasma membrane channel pannexin-1, indicating that the intracellular decrease of the apoptotic cell is a ‘programmed’ process. Apoptotic cells deficient of pannexin-1 sustained the ability to produce ATP through glycolysis and to consume ATP, and did not stop wasting glucose much longer period than normal apoptotic cells. Thus, the pannexin-1 plays a role in arresting the metabolic activity of dead apoptotic cells, most likely through facilitating the loss of intracellular ATP. |
記述: | 死にゆく細胞のATP濃度変化を詳細に可視化することに成功 --積極的にATP濃度を下げる因子を明らかに--. 京都大学プレスリリース. 2020-10-22. |
著作権等: | Copyright Imamura et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
URI: | http://hdl.handle.net/2433/255618 |
DOI(出版社版): | 10.7554/eLife.61960 |
PubMed ID: | 33052098 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2020-10-22 |
出現コレクション: | 学術雑誌掲載論文等 |
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