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dc.contributor.authorSugiura, Harukaen
dc.contributor.authorNagase, Ayumien
dc.contributor.authorOiki, Sayokoen
dc.contributor.authorMikami, Bunzoen
dc.contributor.authorWatanabe, Daisukeen
dc.contributor.authorHashimoto, Wataruen
dc.contributor.alternative杉浦, 春香ja
dc.contributor.alternative長瀬, 鮎美ja
dc.contributor.alternative老木, 紗予子ja
dc.contributor.alternative三上, 文三ja
dc.contributor.alternative渡邉, 大輔ja
dc.contributor.alternative橋本, 渉ja
dc.description大豆と納豆菌のせめぎ合いの仕組みを解明 --生きた大豆は納豆菌を嫌い、納豆菌は死んだ大豆が好き--. 京都大学プレスリリース. 2020-11-02.ja
dc.description.abstractSaprophytic bacteria and plants compete for limited nutrient sources. Bacillus subtilis grows well on steamed soybeans Glycine max to produce the fermented food, natto. Here we focus on bacterial responses in conflict between B. subtilis and G. max. B. subtilis cells maintained high growth rates specifically on non-germinating, dead soybean seeds. On the other hand, viable soybean seeds with germinating capability attenuated the initial growth of B. subtilis. Thus, B. subtilis cells may trigger saprophytic growth in response to the physiological status of G. max. Scanning electron microscope observation indicated that B. subtilis cells on steamed soybeans undergo morphological changes to form apertures, demonstrating cell remodeling during saprophytic growth. Further, transcriptomic analysis of B. subtilis revealed upregulation of the gene cluster, yesOPQR, in colonies growing on steamed soybeans. Recombinant YesO protein, a putative, solute-binding protein for the ATP-binding cassette transporter system, exhibited an affinity for pectin-derived oligosaccharide from plant cell wall. The crystal structure of YesO, in complex with the pectin oligosaccharide, was determined at 1.58 Å resolution. This study expands our knowledge of defensive and offensive strategies in interspecies competition, which may be promising targets for crop protection and fermented food production.en
dc.publisherSpringer Natureen
dc.rights© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit
dc.subjectMolecular biologyen
dc.subjectStructural biologyen
dc.titleBacterial inducible expression of plant cell wall-binding protein YesO through conflict between Glycine max and saprophytic Bacillus subtilisen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleScientific Reportsen
dcterms.accessRightsopen access-
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