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| タイトル: | Increased predominance of the matured ventricular subtype in embryonic stem cell-derived cardiomyocytes in vivo |
| 著者: | Ichimura, Hajime Kadota, Shin Kashihara, Toshihide Yamada, Mitsuhiko Ito, Kuniaki Kobayashi, Hideki Tanaka, Yuki Shiba, Naoko Chuma, Shinichiro   Tohyama, Shugo Seto, Tatsuichiro Okada, Kenji Kuwahara, Koichiro Shiba, Yuji |
| 著者名の別形: | 市村, 創 門田, 真 柏原, 俊英 山田, 充彦 小林, 秀樹 田中, 夕祈 柴, 直子 中馬, 新一郎 遠山, 周吾 瀬戸, 達一郎 岡田, 健次 桑原, 宏一郎 柴, 祐司 |
| キーワード: | Embryonic stem cells Regeneration |
| 発行日: | 17-Jul-2020 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 10 |
| 論文番号: | 11883 |
| 抄録: | Accumulating evidence suggests that human pluripotent stem cell-derived cardiomyocytes can affect “heart regeneration”, replacing injured cardiac scar tissue with concomitant electrical integration. However, electrically coupled graft cardiomyocytes were found to innately induce transient post-transplant ventricular tachycardia in recent large animal model transplantation studies. We hypothesised that these phenomena were derived from alterations in the grafted cardiomyocyte characteristics. In vitro experiments showed that human embryonic stem cell-derived cardiomyocytes (hESC-CMs) contain nodal-like cardiomyocytes that spontaneously contract faster than working-type cardiomyocytes. When transplanted into athymic rat hearts, proliferative capacity was lower for nodal-like than working-type cardiomyocytes with grafted cardiomyocytes eventually comprising only relatively matured ventricular cardiomyocytes. RNA-sequencing of engrafted hESC-CMs confirmed the increased expression of matured ventricular cardiomyocyte-related genes, and simultaneous decreased expression of nodal cardiomyocyte-related genes. Temporal engraftment of electrical excitable nodal-like cardiomyocytes may thus explain the transient incidence of post-transplant ventricular tachycardia, although further large animal model studies will be required to control post-transplant arrhythmia. |
| 著作権等: | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/255869 |
| DOI(出版社版): | 10.1038/s41598-020-68373-9 |
| PubMed ID: | 32681032 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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