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dc.contributor.author | Tamura, Tomonori | en |
dc.contributor.author | Fujisawa, Alma | en |
dc.contributor.author | Tsuchiya, Masaki | en |
dc.contributor.author | Shen, Yuying | en |
dc.contributor.author | Nagao, Kohjiro | en |
dc.contributor.author | Kawano, Shin | en |
dc.contributor.author | Tamura, Yasushi | en |
dc.contributor.author | Endo, Toshiya | en |
dc.contributor.author | Umeda, Masato | en |
dc.contributor.author | Hamachi, Itaru | en |
dc.contributor.alternative | 田村, 朋則 | ja |
dc.contributor.alternative | 藤沢, 有磨 | ja |
dc.contributor.alternative | 土谷, 正樹 | ja |
dc.contributor.alternative | 沈, 佑穎 | ja |
dc.contributor.alternative | 長尾, 耕治郎 | ja |
dc.contributor.alternative | 河野, 慎 | ja |
dc.contributor.alternative | 田村, 康 | ja |
dc.contributor.alternative | 遠藤, 斗志也 | ja |
dc.contributor.alternative | 梅田, 真郷 | ja |
dc.contributor.alternative | 濵地, 格 | ja |
dc.date.accessioned | 2020-11-25T05:18:54Z | - |
dc.date.available | 2020-11-25T05:18:54Z | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.issn | 1552-4469 | - |
dc.identifier.uri | http://hdl.handle.net/2433/259230 | - |
dc.description | 狙った細胞内小器官脂質の可視化に成功 --オートファゴソーム形成機構解明に貢献--. 京都大学プレスリリース. 2020-09-28. | ja |
dc.description.abstract | Lipids play crucial roles as structural elements, signaling molecules and material transporters in cells. However, the functions and dynamics of lipids within cells remain unclear because of a lack of methods to selectively label lipids in specific organelles and trace their movement by live-cell imaging. We describe here a technology for the selective labeling and fluorescence imaging (microscopic or nanoscopic) of phosphatidylcholine in target organelles. This approach involves the metabolic incorporation of azido-choline, followed by a spatially limited bioorthogonal reaction that enables the visualization and quantitative analysis of interorganelle lipid transport in live cells. More importantly, with live-cell imaging, we obtained direct evidence that the autophagosomal membrane originates from the endoplasmic reticulum. This method is simple and robust and is thus powerful for real-time tracing of interorganelle lipid trafficking. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | This is the accepted manuscript of the article, which has been published in final form at https://doi.org/10.1038/s41589-020-00651-z. | en |
dc.rights | The full-text file will be made open to the public on 21 March 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Chemical tools | en |
dc.subject | Imaging | en |
dc.subject | Membrane trafficking | en |
dc.subject | Phospholipids | en |
dc.title | Organelle membrane-specific chemical labeling and dynamic imaging in living cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Nature Chemical Biology | en |
dc.identifier.volume | 16 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 1361 | - |
dc.identifier.epage | 1367 | - |
dc.relation.doi | 10.1038/s41589-020-00651-z | - |
dc.textversion | author | - |
dc.address | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University・JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience | en |
dc.address | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University・JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience | en |
dc.address | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University・JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience | en |
dc.address | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University | en |
dc.address | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University | en |
dc.address | Faculty of Life Sciences, Kyoto Sangyo University・Institute for Protein Dynamics, Kyoto Sangyo University | en |
dc.address | Faculty of Science, Yamagata University | en |
dc.address | Faculty of Life Sciences, Kyoto Sangyo University・Institute for Protein Dynamics, Kyoto Sangyo University | en |
dc.address | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University | en |
dc.address | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University・JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience | en |
dc.identifier.pmid | 32958953 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2020-09-28 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2021-03-21 | - |
datacite.awardNumber | 18K14334 | - |
datacite.awardNumber | 19H05764 | - |
datacite.awardNumber | 17H06348 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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