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dc.contributor.author | Matsuda, Hinako | en |
dc.contributor.author | Nakayasu, Masaru | en |
dc.contributor.author | Aoki, Yuichi | en |
dc.contributor.author | Yamazaki, Shinichi | en |
dc.contributor.author | Nagano, Atsushi J. | en |
dc.contributor.author | Yazaki, Kazufumi | en |
dc.contributor.author | Sugiyama, Akifumi | en |
dc.contributor.alternative | 松田, 陽菜子 | ja |
dc.contributor.alternative | 中安, 大 | ja |
dc.contributor.alternative | 矢﨑, 一史 | ja |
dc.contributor.alternative | 杉山, 暁史 | ja |
dc.date.accessioned | 2020-12-16T04:29:37Z | - |
dc.date.available | 2020-12-16T04:29:37Z | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 2475-4455 | - |
dc.identifier.uri | http://hdl.handle.net/2433/259809 | - |
dc.description.abstract | Isoflavones and soyasaponins are major specialized metabolites accumulated in soybean roots and secreted into the rhizosphere. Unlike the biosynthetic pathway, the transporters involved in metabolite secretion remain unknown. The developmental regulation of isoflavone and soyasaponin secretions has been recently reported, but the diurnal regulation of their biosynthesis and secretion still needs to be further studied. To address these challenges, we conducted transcriptome and metabolite analysis using hydroponically grown soybean plants at 6-hr intervals for 48 hr in a 12-hr-light/12-hr-dark condition. Isoflavone and soyasaponin biosynthetic genes showed opposite patterns in the root tissues; that is, the former genes are highly expressed in the daytime, while the latter ones are strongly induced at nighttime. GmMYB176 encoding a transcription factor of isoflavone biosynthesis was upregulated from ZT0 (6:00 a.m.) to ZT6 (12:00 a.m.), followed by the induction of isoflavone biosynthetic genes at ZT6. The isoflavone aglycone content in the roots accordingly increased from ZT6 to ZT18 (0:00 a.m.). The isoflavone aglycone content in root exudates was kept consistent throughout the day, whereas that of glucosides increased at ZT6, which reflected the decreased expression of the gene encoding beta-glucosidase involved in the hydrolysis of apoplast-localized isoflavone conjugates. Co-expression analysis revealed that those isoflavone and soyasaponin biosynthetic genes formed separate clusters, which exhibited a correlation to ABC and MATE transporter genes. In summary, the results in this study indicated the diurnal regulation of isoflavone biosynthesis in soybean roots and the putative transporter genes responsible for isoflavone and soyasaponin transport. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley | en |
dc.rights | © 2020 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd | en |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. | en |
dc.subject | diurnal variability | en |
dc.subject | isoflavones | en |
dc.subject | soyasaponins | en |
dc.subject | soybean roots | en |
dc.subject | transcriptome analysis transporters | en |
dc.title | Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Plant direct | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 11 | - |
dc.relation.doi | 10.1002/pld3.286 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e00286 | - |
dc.identifier.pmid | 33241173 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 18H02313 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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