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タイトル: | Seed-coat protective neolignans are produced by the dirigent protein AtDP1 and the laccase AtLAC5 in Arabidopsis |
著者: | Yonekura-Sakakibara, Keiko Yamamura, Masaomi Matsuda, Fumio Ono, Eiichiro Nakabayashi, Ryo Sugawara, Satoko Mori, Tetsuya Tobimatsu, Yuki https://orcid.org/0000-0002-7578-7392 (unconfirmed) Umezawa, Toshiaki Saito, Kazuki |
著者名の別形: | 榊原, 圭子 山村, 正臣 松田, 史生 小埜, 栄一郎 中林, 亮 菅原, 聡子 森, 哲哉 飛松, 裕基 梅澤, 俊明 斉藤, 和季 |
発行日: | Jan-2021 |
出版者: | Oxford University Press (OUP) |
誌名: | The Plant Cell |
巻: | 33 |
号: | 1 |
開始ページ: | 129 |
終了ページ: | 152 |
抄録: | Lignans/neolignans are generally synthesized from coniferyl alcohol (CA) in the cinnamate/monolignol pathway by oxidation to generate the corresponding radicals with subsequent stereoselective dimerization aided by dirigent proteins (DIRs). Genes encoding oxidases and DIRs for neolignan biosynthesis have not been identified previously. In Arabidopsis thaliana, the DIR AtDP1/AtDIR12 plays an essential role in the 8-O-4′ coupling in neolignan biosynthesis by unequivocal structural determination of the compound missing in the atdp1 mutant as a sinapoylcholine (SC)-conjugated neolignan, erythro-3-{4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-hydroxymethylethoxy]-3, 5-dimethoxyphenyl}acryloylcholine. Phylogenetic analyses showed that AtDP1/AtDIR12 belongs to the DIR-a subfamily composed of DIRs for 8-8′ coupling of monolignol radicals. AtDP1/AtDIR12 is specifically expressed in outer integument 1 cells in developing seeds. As a putative oxidase for neolignan biosynthesis, we focused on AtLAC5, a laccase gene coexpressed with AtDP1/AtDIR12. In lac5 mutants, the abundance of feruloylcholine (FC)-conjugated neolignans decreased to a level comparable to those in the atdp1 mutant. In addition, SC/FC-conjugated neolignans were missing in the seeds of mutants defective in SCT/SCPL19, an enzyme that synthesizes SC. These results strongly suggest that AtDP1/AtDIR12 and AtLAC5 are involved in neolignan biosynthesis via SC/FC. A tetrazolium penetration assay showed that seed coat permeability increased in atdp1 mutants, suggesting a protective role of neolignans in A. thaliana seeds. |
記述: | 種子を保護するネオリグナンの生合成機構を解明 --新たな薬効成分の創出に期待--. 京都大学プレスリリース. 2020-12-03. |
著作権等: | © The Author(s) 2020. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/261032 |
DOI(出版社版): | 10.1093/plcell/koaa014 |
PubMed ID: | 33751095 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2020-12-03-1 |
出現コレクション: | 学術雑誌掲載論文等 |
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