ダウンロード数: 244
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
anie.202005887.pdf | 1.14 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Sakamoto, Kentarou | - |
dc.contributor.author | Akishiba, Misao | - |
dc.contributor.author | Iwata, Takahiro | - |
dc.contributor.author | Murata, Kazuya | - |
dc.contributor.author | Mizuno, Seiya | - |
dc.contributor.author | Kawano, Kenichi | - |
dc.contributor.author | Imanishi, Miki | - |
dc.contributor.author | Sugiyama, Fumihiro | - |
dc.contributor.author | Futaki, Shiroh | - |
dc.contributor.alternative | 坂本, 健太郎 | - |
dc.contributor.alternative | 岩田, 恭宗 | - |
dc.contributor.alternative | 今西, 未来 | - |
dc.contributor.alternative | 河野, 健一 | - |
dc.contributor.alternative | 二木, 史朗 | - |
dc.date.accessioned | 2021-02-02T06:15:56Z | - |
dc.date.available | 2021-02-02T06:15:56Z | - |
dc.date.issued | 2020-11-02 | - |
dc.identifier.issn | 1433-7851 | - |
dc.identifier.issn | 1521-3773 | - |
dc.identifier.uri | http://hdl.handle.net/2433/261198 | - |
dc.description.abstract | Endocytic pathways are practical routes for the intracellular delivery of biomacromolecules. Along with this, effective strategies for endosomal cargo release into the cytosol are desired to achieve successful delivery. Focusing on compositional differences between the cell and endosomal membranes and the pH decrease within endosomes, we designed the lipid-sensitive and pH-responsive endosome-lytic peptide HAad. This peptide contains aminoadipic acid (Aad) residues, which serve as a safety catch for preferential permeabilization of endosomal membranes over cell membranes, and His-to-Ala substitutions enhance the endosomolytic activity. The ability of HAad to destabilize endosomal membranes was supported by model studies using large unilamellar vesicles (LUVs) and by increased intracellular delivery of biomacromolecules (including antibodies) into live cells. Cerebral ventricle injection of Cre recombinase with HAad led to Cre/loxP recombination in a mouse model, thus demonstrating potential applicability of HAad in vivo. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley | - |
dc.rights | This is the peer reviewed version of the following article: K. Sakamoto, M. Akishiba, T. Iwata, K. Murata, S. Mizuno, K. Kawano, M. Imanishi, F. Sugiyama, S. Futaki, Angew. Chem. Int. Ed. 2020, 59, 19990, which has been published in final form at https://doi.org/10.1002/anie.202005887. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | - |
dc.rights | The full-text file will be made open to the public on 20 August 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. | - |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject | cationic peptides | - |
dc.subject | cell-penetrating peptides | - |
dc.subject | cytosolic delivery | - |
dc.subject | endosomal escape | - |
dc.subject | protein delivery | - |
dc.title | Optimizing Charge Switching in Membrane Lytic Peptides for Endosomal Release of Biomacromolecules. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Angewandte Chemie International Edition | en |
dc.identifier.volume | 59 | - |
dc.identifier.issue | 45 | - |
dc.identifier.spage | 19990 | - |
dc.identifier.epage | 19998 | - |
dc.relation.doi | 10.1002/anie.202005887 | - |
dc.textversion | author | - |
dc.address | Institute for Chemical Research, Kyoto University | - |
dc.address | Institute for Chemical Research, Kyoto University | - |
dc.address | Institute for Chemical Research, Kyoto University | - |
dc.address | Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba | - |
dc.address | Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba | - |
dc.address | Institute for Chemical Research, Kyoto University | - |
dc.address | Institute for Chemical Research, Kyoto University | - |
dc.address | Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba | - |
dc.address | Institute for Chemical Research, Kyoto University | - |
dc.identifier.pmid | 32557993 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2021-08-20 | - |
datacite.awardNumber | 18H04403 | - |
datacite.awardNumber | 18H04017 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。