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lsa.202000974.pdf | 4.77 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Kojima, Yoji | - |
dc.contributor.author | Yamashiro, Chika | - |
dc.contributor.author | Murase, Yusuke | - |
dc.contributor.author | Yabuta, Yukihiro | - |
dc.contributor.author | Okamoto, Ikuhiro | - |
dc.contributor.author | Iwatani, Chizuru | - |
dc.contributor.author | Tsuchiya, Hideaki | - |
dc.contributor.author | Nakaya, Masataka | - |
dc.contributor.author | Tsukiyama, Tomoyuki | - |
dc.contributor.author | Nakamura, Tomonori | - |
dc.contributor.author | Yamamoto, Takuya | - |
dc.contributor.author | Saitou, Mitinori | - |
dc.contributor.alternative | 小島, 洋児 | - |
dc.contributor.alternative | 山城, 知佳 | - |
dc.contributor.alternative | 村瀬, 佑介 | - |
dc.contributor.alternative | 藪田, 幸宏 | - |
dc.contributor.alternative | 岡本, 郁弘 | - |
dc.contributor.alternative | 岩谷, 千鶴 | - |
dc.contributor.alternative | 土屋, 英明 | - |
dc.contributor.alternative | 中家, 雅隆 | - |
dc.contributor.alternative | 築山, 智之 | - |
dc.contributor.alternative | 中村, 友紀 | - |
dc.contributor.alternative | 山本, 拓也 | - |
dc.contributor.alternative | 斎藤, 通紀 | - |
dc.date.accessioned | 2021-03-05T10:32:31Z | - |
dc.date.available | 2021-03-05T10:32:31Z | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 2575-1077 | - |
dc.identifier.uri | http://hdl.handle.net/2433/261882 | - |
dc.description | ヒト生殖細胞の運命決定機序を解明 --転写因子のみによる生殖細胞の誘導. 京都大学プレスリリース. 2021-03-01. | - |
dc.description | Master regulator for human germ cell specification. 京都大学プレスリリース. 2021-03-01. | - |
dc.description.abstract | The in vitro reconstitution of human germ-cell development provides a robust framework for clarifying key underlying mechanisms. Here, we explored transcription factors (TFs) that engender the germ-cell fate in their pluripotent precursors. Unexpectedly, SOX17, TFAP2C, and BLIMP1, which act under the BMP signaling and are indispensable for human primordial germ-cell-like cell (hPGCLC) specification, failed to induce hPGCLCs. In contrast, GATA3 or GATA2, immediate BMP effectors, combined with SOX17 and TFAP2C, generated hPGCLCs. GATA3/GATA2 knockouts dose-dependently impaired BMP-induced hPGCLC specification, whereas GATA3/GATA2 expression remained unaffected in SOX17, TFAP2C, or BLIMP1 knockouts. In cynomolgus monkeys, a key model for human development, GATA3, SOX17, and TFAP2C were co-expressed exclusively in early PGCs. Crucially, the TF-induced hPGCLCs acquired a hallmark of bona fide hPGCs to undergo epigenetic reprogramming and mature into oogonia/gonocytes in xenogeneic reconstituted ovaries. By uncovering a TF circuitry driving the germ line program, our study provides a paradigm for TF-based human gametogenesis. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Life Science Alliance, LLC | - |
dc.rights | © 2021 Kojima et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). | - |
dc.title | GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Life Science Alliance | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 5 | - |
dc.relation.doi | 10.26508/lsa.202000974 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e202000974 | - |
dc.identifier.pmid | 33608411 | - |
dc.relation.url | https://ashbi.kyoto-u.ac.jp/ja/news/20210219_research-result_saitou/ | - |
dc.relation.url | https://ashbi.kyoto-u.ac.jp/news/20210219_research-result_saitou/ | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 15K08267 | - |
datacite.awardNumber | 20K06654 | - |
datacite.awardNumber | 17H06098 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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