Downloads: 31

Files in This Item:
File Description SizeFormat 
eLife.61092.pdf1.99 MBAdobe PDFView/Open
Title: Retrograde ERK activation waves drive base-to-apex multicellular flow in murine cochlear duct morphogenesis
Authors: Ishii, Mamoru
Tateya, Tomoko
Matsuda, Michiyuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Hirashima, Tsuyoshi  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 石井, 衛
楯谷, 智子
松田, 道行
平島, 剛志
Issue Date: 2021
Publisher: eLife Sciences Publications, Ltd
Journal title: eLife
Volume: 10
Thesis number: e61092
Abstract: A notable example of spiral architecture in organs is the mammalian cochlear duct, where the morphology is critical for hearing function. Genetic studies have revealed necessary signaling molecules, but it remains unclear how cellular dynamics generate elongating, bending, and coiling of the cochlear duct. Here, we show that extracellular signal-regulated kinase (ERK) activation waves control collective cell migration during the murine cochlear duct development using deep tissue live-cell imaging, Förster resonance energy transfer (FRET)-based quantitation, and mathematical modeling. Long-term FRET imaging reveals that helical ERK activation propagates from the apex duct tip concomitant with the reverse multicellular flow on the lateral side of the developing cochlear duct, resulting in advection-based duct elongation. Moreover, model simulations, together with experiments, explain that the oscillatory wave trains of ERK activity and the cell flow are generated by mechanochemical feedback. Our findings propose a regulatory mechanism to coordinate the multicellular behaviors underlying the duct elongation during development.
Description: うずまき管の伸⻑を司る分子活性と細胞群の波を発見 --綱引きによる細胞群の流れと臓器の成長--. 京都大学プレスリリース. 2021-03-09.
Rights: © 2021, Ishii et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
DOI(Published Version): 10.7554/eLife.61092
PubMed ID: 33667159
Related Link:
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks

Export Format: 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.