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j.gastro.2021.02.019.pdf | 3.52 MB | Adobe PDF | 見る/開く |
タイトル: | Identification of an Anti–Integrin αvβ6 Autoantibody in Patients With Ulcerative Colitis |
著者: | Kuwada, Takeshi Shiokawa, Masahiro Kodama, Yuzo Ota, Sakiko Kakiuchi, Nobuyuki https://orcid.org/0000-0003-4893-5414 (unconfirmed) Nannya, Yasuhito Yamazaki, Hajime https://orcid.org/0000-0002-9034-4370 (unconfirmed) Yoshida, Hiroyuki Nakamura, Takeharu Matsumoto, Shimpei Muramoto, Yuya Yamamoto, Shuji Honzawa, Yusuke Kuriyama, Katsutoshi Okamoto, Kanako Hirano, Tomonori Okada, Hirokazu Marui, Saiko Sogabe, Yuko Morita, Toshihiro Matsumori, Tomoaki Mima, Atsushi Nishikawa, Yoshihiro https://orcid.org/0000-0002-1113-6937 (unconfirmed) Ueda, Tatsuki Matsumura, Kazuyoshi Uza, Norimitsu Chiba, Tsutomu Seno, Hiroshi |
著者名の別形: | 桒田, 威 塩川, 雅広 児玉, 裕三 太田, 彩貴子 垣内, 伸之 南谷, 泰仁 山崎, 大 中村, 武晴 村本, 雄哉 山本, 修司 本澤, 有介 栗山, 勝利 岡田, 浩和 丸井, 彩子 森田, 敏広 松森, 友昭 西川, 義浩 松村, 和宜 宇座, 徳光 千葉, 勉 妹尾, 浩 |
キーワード: | Inflammatory Bowel Disease Autoimmunity Epithelial Adhesion Molecule RGD Motif |
発行日: | Jul-2021 |
出版者: | Elsevier BV |
誌名: | Gastroenterology |
巻: | 160 |
号: | 7 |
開始ページ: | 2383 |
終了ページ: | 2394 |
論文番号: | e21 |
抄録: | Background and Aims: Ulcerative colitis is the most frequent type of inflammatory bowel disease and is characterized by colonic epithelial cell damage. Although involvement of autoimmunity has been suggested in ulcerative colitis, specific autoantigens/antibodies have yet to be elucidated. Methods: Using 23 recombinant integrin proteins, we performed enzyme-linked immunosorbent assays on sera from patients with ulcerative colitis and controls. Integrin expression and IgG binding in the colon tissues of patients with ulcerative colitis and controls were examined using immunofluorescence and coimmunoprecipitation, respectively. The blocking activity of autoantibodies was examined using solid-phase binding and cell adhesion assays. Results: Screening revealed that patients with ulcerative colitis had IgG antibodies against integrin αvβ6. In the training and validation groups, 103 of 112 (92.0%) patients with ulcerative colitis and only 8 of 155 (5.2%) controls had anti–integrin αvβ6 antibodies (P < .001), resulting in a sensitivity of 92.0% and a specificity of 94.8% for diagnosing ulcerative colitis. Anti–integrin αvβ6 antibody titers coincided with ulcerative colitis disease activity, and IgG1 was the major subclass. Patient IgG bound to the integrin αvβ6 expressed on colonic epithelial cells. Moreover, IgG of patients with ulcerative colitis blocked integrin αvβ6–fibronectin binding through an RGD (Arg-Gly-Asp) tripeptide motif and inhibited cell adhesion. Conclusions: A significant majority of patients with ulcerative colitis had autoantibodies against integrin αvβ6, which may serve as a potential diagnostic biomarker with high sensitivity and specificity. |
記述: | 指定難病「潰瘍性大腸炎」の自己抗体発見 --新たな診断や治療開発へ--. 京都大学プレスリリース. 2021-03-09. |
著作権等: | © 2021 by the AGA Institute. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/261943 |
DOI(出版社版): | 10.1053/j.gastro.2021.02.019 |
PubMed ID: | 33582126 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2021-03-09-1 |
出現コレクション: | 学術雑誌掲載論文等 |
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