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タイトル: Identification of an Anti–Integrin αvβ6 Autoantibody in Patients With Ulcerative Colitis
著者: Kuwada, Takeshi
Shiokawa, Masahiro  kyouindb  KAKEN_id
Kodama, Yuzo
Ota, Sakiko
Kakiuchi, Nobuyuki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-4893-5414 (unconfirmed)
Nannya, Yasuhito  KAKEN_id
Yamazaki, Hajime  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-9034-4370 (unconfirmed)
Yoshida, Hiroyuki
Nakamura, Takeharu
Matsumoto, Shimpei
Muramoto, Yuya
Yamamoto, Shuji  KAKEN_id
Honzawa, Yusuke
Kuriyama, Katsutoshi
Okamoto, Kanako
Hirano, Tomonori
Okada, Hirokazu
Marui, Saiko
Sogabe, Yuko
Morita, Toshihiro
Matsumori, Tomoaki
Mima, Atsushi
Nishikawa, Yoshihiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-1113-6937 (unconfirmed)
Ueda, Tatsuki
Matsumura, Kazuyoshi
Uza, Norimitsu  KAKEN_id
Chiba, Tsutomu
Seno, Hiroshi  kyouindb  KAKEN_id
著者名の別形: 桒田, 威
塩川, 雅広
児玉, 裕三
太田, 彩貴子
垣内, 伸之
南谷, 泰仁
山崎, 大
中村, 武晴
村本, 雄哉
山本, 修司
本澤, 有介
栗山, 勝利
岡田, 浩和
丸井, 彩子
森田, 敏広
松森, 友昭
西川, 義浩
松村, 和宜
宇座, 徳光
千葉, 勉
妹尾, 浩
キーワード: Inflammatory Bowel Disease
Autoimmunity
Epithelial Adhesion Molecule
RGD Motif
発行日: Jul-2021
出版者: Elsevier BV
誌名: Gastroenterology
巻: 160
号: 7
開始ページ: 2383
終了ページ: 2394
論文番号: e21
抄録: Background and Aims: Ulcerative colitis is the most frequent type of inflammatory bowel disease and is characterized by colonic epithelial cell damage. Although involvement of autoimmunity has been suggested in ulcerative colitis, specific autoantigens/antibodies have yet to be elucidated. Methods: Using 23 recombinant integrin proteins, we performed enzyme-linked immunosorbent assays on sera from patients with ulcerative colitis and controls. Integrin expression and IgG binding in the colon tissues of patients with ulcerative colitis and controls were examined using immunofluorescence and coimmunoprecipitation, respectively. The blocking activity of autoantibodies was examined using solid-phase binding and cell adhesion assays. Results: Screening revealed that patients with ulcerative colitis had IgG antibodies against integrin αvβ6. In the training and validation groups, 103 of 112 (92.0%) patients with ulcerative colitis and only 8 of 155 (5.2%) controls had anti–integrin αvβ6 antibodies (P < .001), resulting in a sensitivity of 92.0% and a specificity of 94.8% for diagnosing ulcerative colitis. Anti–integrin αvβ6 antibody titers coincided with ulcerative colitis disease activity, and IgG1 was the major subclass. Patient IgG bound to the integrin αvβ6 expressed on colonic epithelial cells. Moreover, IgG of patients with ulcerative colitis blocked integrin αvβ6–fibronectin binding through an RGD (Arg-Gly-Asp) tripeptide motif and inhibited cell adhesion. Conclusions: A significant majority of patients with ulcerative colitis had autoantibodies against integrin αvβ6, which may serve as a potential diagnostic biomarker with high sensitivity and specificity.
記述: 指定難病「潰瘍性大腸炎」の自己抗体発見 --新たな診断や治療開発へ--. 京都大学プレスリリース. 2021-03-09.
著作権等: © 2021 by the AGA Institute. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/261943
DOI(出版社版): 10.1053/j.gastro.2021.02.019
PubMed ID: 33582126
関連リンク: https://www.kyoto-u.ac.jp/ja/research-news/2021-03-09-1
出現コレクション:学術雑誌掲載論文等

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