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タイトル: | Evolutionary Selection of the Nuclear Localization Signal in the Viral Nucleoprotein Leads to Host Adaptation of the Genus Orthobornavirus. |
著者: | Komorizono, Ryo https://orcid.org/0000-0002-7610-7985 (unconfirmed) Sassa, Yukiko Horie, Masayuki Makino, Akiko Tomonaga, Keizo https://orcid.org/0000-0003-0405-7103 (unconfirmed) |
著者名の別形: | 小森園, 亮 佐々, 悠木子 堀江, 真行 牧野, 晶子 朝長, 啓造 |
キーワード: | bornavirus host adaptation viral evolution nuclear transport |
発行日: | Nov-2020 |
出版者: | MDPI AG |
誌名: | Viruses |
巻: | 12 |
号: | 11 |
抄録: | Adaptation of the viral life cycle to host cells is necessary for efficient viral infection and replication. This evolutionary process has contributed to the mechanism for determining the host range of viruses. Orthobornaviruses, members of the family Bornaviridae, are non-segmented, negative-strand RNA viruses, and several genotypes have been isolated from different vertebrate species. Previous studies revealed that some genotypes isolated from avian species can replicate in mammalian cell lines, suggesting the zoonotic potential of avian orthobornaviruses. However, the mechanism by which the host specificity of orthobornaviruses is determined has not yet been identified. In this study, we found that the infectivity of orthobornaviruses is not determined at the viral entry step, mediated by the viral glycoprotein and matrix protein. Furthermore, we demonstrated that the nuclear localization signal (NLS) sequence in the viral nucleoprotein (N) has evolved under natural selection and determines the host-specific viral polymerase activity. A chimeric mammalian orthobornavirus, which has the NLS sequence of avian orthobornavirus N, exhibited a reduced propagation efficiency in mammalian cells. Our findings indicated that nuclear transport of the viral N is a determinant of the host range of orthobornaviruses, providing insights into the evolution and host adaptation of orthobornaviruses. |
著作権等: | © 2020 by the authors Licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license |
URI: | http://hdl.handle.net/2433/262590 |
DOI(出版社版): | 10.3390/v12111291 |
PubMed ID: | 33187187 |
出現コレクション: | 学術雑誌掲載論文等 |
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