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DCフィールド | 値 | 言語 |
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dc.contributor.author | Sato, Shinya | en |
dc.contributor.author | Yamashita, Takahiro | en |
dc.contributor.author | Matsuda, Michiyuki | en |
dc.contributor.alternative | 佐藤, 慎哉 | ja |
dc.contributor.alternative | 山下, 高廣 | ja |
dc.contributor.alternative | 松田, 道行 | ja |
dc.date.accessioned | 2021-07-19T00:28:16Z | - |
dc.date.available | 2021-07-19T00:28:16Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.uri | http://hdl.handle.net/2433/264443 | - |
dc.description | 網膜の知られざる光応答を顕微鏡観察で発見 --光センサー細胞が暗黒に反応した--. 京都大学プレスリリース. 2020-10-14. | ja |
dc.description | Detect with PKAchu. 京都大学プレスリリース. 2020-10-30. | en |
dc.description.abstract | Light-induced extrasynaptic dopamine release in the retina reduces adenosine 3′, 5′-cyclic monophosphate (cAMP) in rod photoreceptor cells, which is thought to mediate light-dependent desensitization. However, the fine time course of the cAMP dynamics in rods remains elusive due to technical difficulty. Here, we visualized the spatiotemporal regulation of cAMP-dependent protein kinase (PKA) in mouse rods by two-photon live imaging of retinal explants of PKAchu mice, which express a fluorescent biosensor for PKA. Unexpectedly, in addition to the light-on-induced suppression, we observed prominent light-off-induced PKA activation. This activation required photopic light intensity and was confined to the illuminated rods. The estimated maximum spectral sensitivity of 489 nm and loss of the light-off-induced PKA activation in rod-transducin-knockout retinas strongly suggest the involvement of rhodopsin. In support of this notion, rhodopsin-deficient retinal explants showed only the light-on-induced PKA suppression. Taken together, these results suggest that, upon photopic light stimulation, rhodopsin and dopamine signals are integrated to shape the light-off-induced cAMP production and following PKA activation. This may support the dark adaptation of rods. | en |
dc.language.iso | eng | - |
dc.publisher | National Academy of Sciences | en |
dc.rights | This is a postprint of the article, which has been published in final form at https://doi.org/10.1073/pnas.2009164117. | en |
dc.rights | This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | en |
dc.subject | retina | en |
dc.subject | two-photon microscopy | en |
dc.subject | FRET biosensor | en |
dc.subject | protein kinase A | en |
dc.subject | rhodopsin | en |
dc.title | Rhodopsin-mediated light-off-induced protein kinase A activation in mouse rod photoreceptor cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Proceedings of the National Academy of Sciences of the United States of America | en |
dc.identifier.volume | 117 | - |
dc.identifier.issue | 43 | - |
dc.identifier.spage | 26996 | - |
dc.identifier.epage | 27003 | - |
dc.relation.doi | 10.1073/pnas.2009164117 | - |
dc.textversion | author | - |
dc.address | Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University | en |
dc.address | Department of Biophysics, Graduate School of Science, Kyoto University | en |
dc.address | Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University; Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University | en |
dc.identifier.pmid | 33046651 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research/research_results/2020/201013_2.html | - |
dc.relation.url | https://www.kyoto-u.ac.jp/en/research/research_results/2020/201013_2.html | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2021-04-27 | - |
datacite.awardNumber | 15H05949 | - |
datacite.awardNumber | 16H06280 | - |
datacite.awardNumber | 19H00993 | - |
datacite.awardNumber | 19K16087 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PLANNED-15H05949/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16H06280/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19H00993/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19K16087/ | - |
dc.identifier.pissn | 0027-8424 | - |
dc.identifier.eissn | 1091-6490 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 細胞間コミュニケーションのライブイメージング | ja |
jpcoar.awardTitle | 先端バイオイメージング支援プラットフォーム | ja |
jpcoar.awardTitle | 細胞増殖因子情報伝達系の活性波による細胞集団移動制御機構 | ja |
jpcoar.awardTitle | 視細胞で光依存的・ロドプシン非依存的に起こるPKA活性変化の生理的意義とは? | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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