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Title: | Core-shell hydrogel microfiber-expanded pluripotent stem cell-derived lung progenitors applicable to lung reconstruction in vivo |
Authors: | Ikeo, Satoshi Yamamoto, Yuki Ikeda, Kazuhiro Sone, Naoyuki https://orcid.org/0000-0002-3378-728X (unconfirmed) Korogi, Yohei Tomiyama, Lucia Matsumoto, Hisako Hirai, Toyohiro Hagiwara, Masatoshi Gotoh, Shimpei |
Author's alias: | 池尾, 聡 山本, 佑樹 池田, 和弘 曽根, 尚之 興梠, 陽平 松本, 久子 平井, 豊博 萩原, 正敏 後藤, 慎平 |
Keywords: | Pluripotent stem cells Lung progenitors Core-shell hydrogel microfibers Three-dimensional cell culture Xenotransplantation Regenerative medicine |
Issue Date: | Sep-2021 |
Publisher: | Elsevier BV |
Journal title: | Biomaterials |
Volume: | 276 |
Thesis number: | 121031 |
Abstract: | Lung transplantation is the only treatment available for end-stage lung diseases; however, donor shortage is a global issue. The use of human pluripotent stem cells (hPSCs) for organ regeneration is a promising approach. Nevertheless, methods for the expansion of isolated hPSC-derived lung progenitors (hLPs) for transplantation purposes have not yet been reported. Herein, we established an expansion system of hLPs based on their three-dimensional culture in core-shell hydrogel microfibers, that ensures the maintenance of their bipotency for differentiation into alveolar and airway epithelial cells including alveolar type II (AT2) cells. Further, we developed an efficient in vivo transplantation method using an endoscope-assisted transtracheal administration system; the successful engraftment and in vivo differentiation of hLPs into alveolar epithelial cells (incorporated into the alveoli) was observed. Importantly, expanded hLPs in the context of microfibers were successfully transplanted into the murine lungs, opening avenues for cell-based therapies of lung diseases. Therefore, our novel method has potential regenerative medicine applications; additionally, the high-quality hLPs and AT2 cells generated via the microfiber-based technology are valuable for drug discovery purposes. |
Description: | ヒトiPS細胞由来肺前駆細胞の拡大培養とマウス肺への移植・生着に成功 --肺再生医療の実現へ大きな一歩--. 京都大学プレスリリース. 2021-07-30. |
Rights: | © 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the Creative Commons Attribution license. |
URI: | http://hdl.handle.net/2433/264609 |
DOI(Published Version): | 10.1016/j.biomaterials.2021.121031 |
PubMed ID: | 34304138 |
Related Link: | https://www.kyoto-u.ac.jp/ja/research-news/2021-07-30-1 |
Appears in Collections: | Journal Articles |
This item is licensed under a Creative Commons License