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Title: Core-shell hydrogel microfiber-expanded pluripotent stem cell-derived lung progenitors applicable to lung reconstruction in vivo
Authors: Ikeo, Satoshi
Yamamoto, Yuki
Ikeda, Kazuhiro
Sone, Naoyuki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3378-728X (unconfirmed)
Korogi, Yohei
Tomiyama, Lucia
Matsumoto, Hisako
Hirai, Toyohiro
Hagiwara, Masatoshi  kyouindb  KAKEN_id
Gotoh, Shimpei
Author's alias: 池尾, 聡
山本, 佑樹
池田, 和弘
曽根, 尚之
興梠, 陽平
松本, 久子
平井, 豊博
萩原, 正敏
後藤, 慎平
Keywords: Pluripotent stem cells
Lung progenitors
Core-shell hydrogel microfibers
Three-dimensional cell culture
Xenotransplantation
Regenerative medicine
Issue Date: Sep-2021
Publisher: Elsevier BV
Journal title: Biomaterials
Volume: 276
Thesis number: 121031
Abstract: Lung transplantation is the only treatment available for end-stage lung diseases; however, donor shortage is a global issue. The use of human pluripotent stem cells (hPSCs) for organ regeneration is a promising approach. Nevertheless, methods for the expansion of isolated hPSC-derived lung progenitors (hLPs) for transplantation purposes have not yet been reported. Herein, we established an expansion system of hLPs based on their three-dimensional culture in core-shell hydrogel microfibers, that ensures the maintenance of their bipotency for differentiation into alveolar and airway epithelial cells including alveolar type II (AT2) cells. Further, we developed an efficient in vivo transplantation method using an endoscope-assisted transtracheal administration system; the successful engraftment and in vivo differentiation of hLPs into alveolar epithelial cells (incorporated into the alveoli) was observed. Importantly, expanded hLPs in the context of microfibers were successfully transplanted into the murine lungs, opening avenues for cell-based therapies of lung diseases. Therefore, our novel method has potential regenerative medicine applications; additionally, the high-quality hLPs and AT2 cells generated via the microfiber-based technology are valuable for drug discovery purposes.
Description: ヒトiPS細胞由来肺前駆細胞の拡大培養とマウス肺への移植・生着に成功 --肺再生医療の実現へ大きな一歩--. 京都大学プレスリリース. 2021-07-30.
Rights: © 2021 The Author(s).
Published by Elsevier Ltd. This is an open access article under the Creative Commons Attribution license.
URI: http://hdl.handle.net/2433/264609
DOI(Published Version): 10.1016/j.biomaterials.2021.121031
PubMed ID: 34304138
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2021-07-30-1
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