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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| anie.202105527.pdf | 1.46 MB | Adobe PDF | 見る/開く |
| タイトル: | Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides |
| 著者: | Iwata, Takahiro Hirose, Hisaaki Sakamoto, Kentarou Hirai, Yusuke Arafiles, Jan Vincent V. Akishiba, Misao Imanishi, Miki    https://orcid.org/0000-0002-4172-0966 (unconfirmed)Futaki, Shiroh |
| 著者名の別形: | 岩田, 恭宗 廣瀨, 久昭 坂本, 健太郎 平井, 勇祐 秋柴, 美沙穂 今西, 未来 二木, 史朗 |
| キーワード: | antibodies intracellular delivery liquid droplet liquid-liquid phase separation (LLPS) peptides |
| 発行日: | Sep-2021 |
| 出版者: | Wiley |
| 誌名: | Angewandte Chemie International Edition |
| 巻: | 60 |
| 号: | 36 |
| 開始ページ: | 19804 |
| 終了ページ: | 19812 |
| 抄録: | Fc region binding peptide conjugated with attenuated cationic amphiphilic lytic peptide L17E trimer [FcB(L17E)₃] was designed for immunoglobulin G (IgG) delivery into cells. Particle-like liquid droplets were generated by mixing Alexa Fluor 488 labeled IgG (Alexa488-IgG) with FcB(L17E)₃. Droplet contact with the cellular membrane led to spontaneous influx and distribution of Alexa488-IgG throughout cells in serum containing medium. Involvement of cellular machinery accompanied by actin polymerization and membrane ruffling was suggested for the translocation. Alexa488-IgG negative charges were crucial in liquid droplet formation with positively charged FcB(L17E)₃. Binding of IgG to FcB(L17E)₃ may not be necessary. Successful intracellular delivery of Alexa Fluor 594-labeled anti-nuclear pore complex antibody and anti-mCherry-nanobody tagged with supernegatively charged green fluorescence protein allowed binding to cellular targets in the presence of FcB(L17E)₃. |
| 記述: | 抗体を液滴に濃縮し細胞内へ高速輸送 --クモ毒改良ペプチドと抗体による液－液相分離の誘起と抗体の細胞内輸送--. 京都大学プレスリリース. 2021-08-06. |
| 著作権等: | This is the peer reviewed version of the following article: [Takahiro Iwata, Hisaaki Hirose, Kentarou Sakamoto, Yusuke Hirai, Jan Vincent V. Arafiles, Misao Akishiba, Miki Imanishi, Shiroh Futaki. Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides. Angewandte Chemie International Edition, 60(36), 19804-19812.], which has been published in final form at https://doi.org/10.1002/anie.202105527]. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. The full-text file will be made open to the public on 9 July 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
| URI: | http://hdl.handle.net/2433/264950 |
| DOI(出版社版): | 10.1002/anie.202105527 |
| PubMed ID: | 34114295 |
| 関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2021-08-06-1 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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