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タイトル: | Two-Photon AMPK and ATP Imaging Reveals Metabolic Recovery in Mouse Rod Photoreceptor Cells |
著者: | He, Jiazhou Yamamoto, Masamichi Sumiyama, Kenta Konagaya, Yumi Terai, Kenta https://orcid.org/0000-0001-7638-3720 (unconfirmed) Matsuda, Michiyuki https://orcid.org/0000-0002-5876-9969 (unconfirmed) Sato, Shinya |
著者名の別形: | 小長谷, 有美 寺井, 健太 松田, 道行 佐藤, 慎哉 |
発行日: | Sep-2021 |
出版者: | Wiley |
誌名: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology |
巻: | 35 |
号: | 9 |
論文番号: | e21880 |
抄録: | In vertebrates, retinal rod and cone photoreceptor cells rely significantly on glycolysis. Lactate released from photoreceptor cells fuels neighboring retinal pigment epithelium cells and Müller glial cells through oxidative phosphorylation. To understand this highly heterogeneous metabolic environment around photoreceptor cells, single-cell analysis is needed. Here, we visualized cellular AMP-activated protein kinase (AMPK) activity and ATP levels in the retina by two-photon microscopy. Transgenic mice expressing a hyBRET-AMPK biosensor were used for measuring the AMPK activity. GO-ATeam2 transgenic mice were used for measuring the ATP level. Temporal metabolic responses were successfully detected in the live retinal explants upon drug perfusion. A glycolysis inhibitor, 2-deoxy-d-glucose (2-DG), activated AMPK and reduced ATP. These effects were clearly stronger in rods than in cones. Notably, rod AMPK and ATP started to recover at 30 min from the onset of 2-DG perfusion. Consistent with these findings, ex vivo electroretinogram recordings showed a transient slowdown in rod dim flash responses during a 60-min 2-DG perfusion, whereas cone responses were not affected. Based on these results, we propose that cones surrounded by highly glycolytic rods become less dependent on glycolysis, and rods also become less dependent on glycolysis within 60 min upon the glycolysis inhibition. |
記述: | The title of publisher's version: Two-photon AMPK and ATP imaging reveals the bias between rods and cones in glycolysis utility. |
著作権等: | This is the original submitted manuscript of following article: 'FASEB Journal' Volume35, Issue9, e21880, https://doi.org/10.1096/fj.202101121R. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/265298 |
DOI(出版社版): | 10.1096/fj.202101121R |
PubMed ID: | 34449091 |
関連リンク: | https://onlinelibrary.wiley.com/share/author/CZPNNXCKDBVED8GRTRMD?target=10.1096/fj.202101121R |
出現コレクション: | 学術雑誌掲載論文等 |
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