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タイトル: | Extracellular vesicles synchronize cellular phenotypes of differentiating cells |
著者: | Minakawa, Tomohiro Matoba, Tetsuya Ishidate, Fumiyoshi Fujiwara, Takahiro K. Takehana, Sho Tabata, Yasuhiko Yamashita, Jun K. |
著者名の別形: | 皆川, 朋皓 的場, 哲哉 石館, 文善 藤原, 敬宏 竹花, 祥 田畑, 泰彦 山下, 潤 |
キーワード: | differentiation embryos miR-132 nanoparticles stem cells synchronization |
発行日: | Sep-2021 |
出版者: | Wiley |
誌名: | Journal of Extracellular Vesicles |
巻: | 10 |
号: | 11 |
論文番号: | e12147 |
抄録: | During embryonic development, cells differentiate in a coordinated manner, aligning their fate decisions and differentiation stages with those of surrounding cells. However, little is known about the mechanisms that regulate this synchrony. Here we show that cells in close proximity synchronize their differentiation stages and cellular phenotypes with each other via extracellular vesicle (EV)-mediated cellular communication. We previously established a mouse embryonic stem cell (ESC) line harbouring an inducible constitutively active protein kinase A (CA-PKA) gene and found that the ESCs rapidly differentiated into mesoderm after PKA activation. In the present study, we performed a co-culture of Control-ESCs and PKA-ESCs, finding that both ESC types rapidly differentiated in synchrony even when PKA was activated only in PKA-ESCs, a phenomenon we named ‘Phenotypic Synchrony of Cells (PSyC)’. We further demonstrated PSyC was mediated by EVs containing miR-132. PKA-ESC-derived EVs and miR-132-containing artificial nano-vesicles similarly enhanced mesoderm and cardiomyocyte differentiation in ESCs and ex vivo embryos, respectively. PSyC is a new form of cell-cell communication mediated by the EV regulation of neighbouring cells and could be broadly involved in tissue development and homeostasis. |
記述: | 細胞外小胞が細胞の分化を同調させる現象の発見. 京都大学プレスリリース. 2021-10-01. Cells act in unison when next to each other. 京都大学プレスリリース. 2021-10-01. |
著作権等: | © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
URI: | http://hdl.handle.net/2433/265343 |
DOI(出版社版): | 10.1002/jev2.12147 |
PubMed ID: | 34533283 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/211001-103000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/211001-103000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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