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1873-3468.12247.pdf | 6.04 MB | Adobe PDF | 見る/開く |
タイトル: | Alteration of transbilayer phospholipid compositions is involved in cell adhesion, cell spreading, and focal adhesion formation |
著者: | Miyano, Rie Matsumoto, Takashi Takatsu, Hiroyuki Nakayama, Kazuhisa https://orcid.org/0000-0001-7701-7183 (unconfirmed) Shin, Hye-Won https://orcid.org/0000-0002-9138-9554 (unconfirmed) |
著者名の別形: | 宮野, 吏永 松本, 尚 高津, 宏之 中山, 和久 申, 惠媛 |
キーワード: | ATPase cell spreading flippase focal adhesion lipid bilayer phospholipid |
発行日: | Jul-2016 |
出版者: | Wiley |
誌名: | FEBS Letters |
巻: | 590 |
号: | 14 |
開始ページ: | 2138 |
終了ページ: | 2145 |
抄録: | We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippases in cell spreading on the extracellular matrix. Expression of PC-flippases, but not PS-flippases, delayed cell adhesion, cell spreading and inhibited formation of focal adhesions. In addition, overexpression of a PS-binding probe that sequesters PS in the cytoplasmic leaflet delayed cell spreading and inhibited formation of focal adhesions. These results suggest that elevation of PC at the cytoplasmic leaflet of the plasma membrane by expression of PC-flippases may reduce the local concentration of PS or phosphoinositides, required for efficient cell adhesion, focal adhesion formation, and cell spreading. |
著作権等: | This is the peer reviewed version of the following article: [FEBS Letters, 590(14), 2138-2145], which has been published in final form at https://doi.org/10.1002/1873-3468.12247. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. The full-text file will be made open to the public on 27 June 2017 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/265375 |
DOI(出版社版): | 10.1002/1873-3468.12247 |
PubMed ID: | 27277390 |
出現コレクション: | 学術雑誌掲載論文等 |
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