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タイトル: | Induction of inverted morphology in brain organoids by vertical-mixing bioreactors |
著者: | Suong, Dang Ngoc Anh Imamura, Keiko Inoue, Ikuyo Kabai, Ryotaro Sakamoto, Satoko Okumura, Tatsuya Kato, Yoshikazu Kondo, Takayuki Yada, Yuichiro Klein, William L. Watanabe, Akira Inoue, Haruhisa https://orcid.org/0000-0003-4736-9537 (unconfirmed) |
著者名の別形: | 今村, 恵子 井上, 育代 樺井, 良太朗 坂本, 智子 奥村, 龍哉 加藤, 好一 近藤, 孝之 矢田, 祐一郎 渡辺, 亮 井上, 治久 |
キーワード: | Stem-cell biotechnology Stem cells |
発行日: | 2021 |
出版者: | Springer Nature |
誌名: | Communications Biology |
巻: | 4 |
論文番号: | 1213 |
抄録: | Organoid technology provides an opportunity to generate brain-like structures by recapitulating developmental steps in the manner of self-organization. Here we examined the vertical-mixing effect on brain organoid structures using bioreactors and established inverted brain organoids. The organoids generated by vertical mixing showed neurons that migrated from the outer periphery to the inner core of organoids, in contrast to orbital mixing. Computational analysis of flow dynamics clarified that, by comparison with orbital mixing, vertical mixing maintained the high turbulent energy around organoids, and continuously kept inter-organoid distances by dispersing and adding uniform rheological force on organoids. To uncover the mechanisms of the inverted structure, we investigated the direction of primary cilia, a cellular mechanosensor. Primary cilia of neural progenitors by vertical mixing were aligned in a multidirectional manner, and those by orbital mixing in a bidirectional manner. Single-cell RNA sequencing revealed that neurons of inverted brain organoids presented a GABAergic character of the ventral forebrain. These results suggest that controlling fluid dynamics by biomechanical engineering can direct stem cell differentiation of brain organoids, and that inverted brain organoids will be applicable for studying human brain development and disorders in the future. |
記述: | 上下動撹拌培養装置を用いた流体制御により誘導した反転型脳オルガノイド 脳オルガノイド誘導の流体シミュレーションと流体力学的理解. 京都大学プレスリリース. 2021-10-27. How you mix cells changes the brain. 京都大学プレスリリース. 2021-10-28. |
著作権等: | © The Author(s) 2021 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/265531 |
DOI(出版社版): | 10.1038/s42003-021-02719-5 |
PubMed ID: | 34686776 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/211027-120000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/211028-110000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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