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acs.nanolett.1c02955.pdf | 6.36 MB | Adobe PDF | 見る/開く |
タイトル: | Microscopic Temperature Control Reveals Cooperative Regulation of Actin–Myosin Interaction by Drebrin E |
著者: | Kubota, Hiroaki Ogawa, Hiroyuki Miyazaki, Makito https://orcid.org/0000-0002-4603-851X (unconfirmed) Ishii, Shuya Oyama, Kotaro Kawamura, Yuki Ishiwata, Shin’ichi Suzuki, Madoka |
著者名の別形: | 久保田, 寛顕 小川, 裕之 宮﨑, 牧人 石井, 秀弥 大山, 廣太郎 川村, 祐貴 石渡, 信一 鈴木, 団 |
キーワード: | actin-binding protein cytoskeleton heat pulse molecular motor motility assay nanofilament regulation temperature imaging |
発行日: | 24-Nov-2021 |
出版者: | American Chemical Society (ACS) |
誌名: | Nano Letters |
巻: | 21 |
号: | 22 |
開始ページ: | 9526 |
終了ページ: | 9533 |
抄録: | Drebrin E is a regulatory protein of intracellular force produced by actomyosin complexes, that is, myosin molecular motors interacting with actin filaments. The expression level of drebrin E in nerve cells decreases as the animal grows, suggesting its pivotal but unclarified role in neuronal development. Here, by applying the microscopic heat pulse method to actomyosin motility assay, the regulatory mechanism is examined from the room temperature up to 37 °C without a thermal denaturing of proteins. We show that the inhibition of actomyosin motility by drebrin E is eliminated immediately and reversibly during heating and depends on drebrin E concentration. The direct observation of quantum dot-labeled drebrin E implies its stable binding to actin filaments during the heat-induced sliding. Our results suggest that drebrin E allosterically modifies the actin filament structure to regulate cooperatively the actomyosin activity at the maintained in vivo body temperature. |
記述: | 胎児の神経を形作る仕組みは精密な温度センサー --母体の体温維持が神経の成熟に重要であることを示唆--. 京都大学プレスリリース. 2021-11-10. |
著作権等: | © 2021 The Authors. Published by American Chemical Society This article is licensed under the Creative Commons Attribution 4.0 International License. |
URI: | http://hdl.handle.net/2433/266182 |
DOI(出版社版): | 10.1021/acs.nanolett.1c02955 |
PubMed ID: | 34751025 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2021-11-10 |
出現コレクション: | 学術雑誌掲載論文等 |
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