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Title: Unique C-terminal region of Hap3 is required for methanol-regulated gene expression in the methylotrophic yeast Candida boidinii
Authors: Oda, Saori
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid (unconfirmed)
Nitta, Nobuhisa
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Author's alias: 小田, 沙織
由里本, 博也
新田, 暢久
阪井, 康能
Issue Date: May-2016
Publisher: Microbiology Society
Journal title: Microbiology
Volume: 162
Issue: 5
Start page: 898
End page: 907
Abstract: The Hap complex of the methylotrophic yeast Candida boidinii was found to be required for methanol-regulated gene expression. In this study, we performed functional characterization of CbHap3p, one of the Hap complex components in C. boidinii. Sequence alignment of Hap3 proteins revealed the presence of a unique extended C-terminal region, which is not present in Hap3p from Saccharomyces cerevisiae (ScHap3p), but is found in Hap3p proteins of methylotrophic yeasts. Deletion of the C-terminal region of CbHap3p (Δ256–292 or Δ107–237) diminished activation of methanol-regulated genes and abolished the ability to grow on methanol, but did not affect nuclear localization or DNA-binding ability. However, deletion of the N-terminal region of CbHap3p (Δ1–20) led to not only a growth defect on methanol and a decreased level of methanol-regulated gene expression, but also impaired nuclear localization and binding to methanol-regulated gene promoters. We also revealed that CbHap3p could complement the growth defect of the Schap3Δ strain on glycerol, although ScHap3p could not complement the growth defect of a Cbhap3Δ strain on methanol. We conclude that the unique C-terminal region of CbHap3p contributes to maximum activation of methanol-regulated genes, whilst the N-terminal region is required for nuclear localization and binding to DNA.
Rights: © Saori Oda, et al., 2016. The definitive peer reviewed, edited version of this article is published in Microbiology, volume 162, issue 5, 2016,
This manuscript is under the Creative Commons Attribution 4.0 International license.
DOI(Published Version): 10.1099/mic.0.000275
PubMed ID: 26963751
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