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Title: Unique C-terminal region of Hap3 is required for methanol-regulated gene expression in the methylotrophic yeast Candida boidinii
Authors: Oda, Saori
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7506-6184 (unconfirmed)
Nitta, Nobuhisa
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Author's alias: 小田, 沙織
由里本, 博也
新田, 暢久
阪井, 康能
Issue Date: May-2016
Publisher: Microbiology Society
Journal title: Microbiology
Volume: 162
Issue: 5
Start page: 898
End page: 907
Abstract: The Hap complex of the methylotrophic yeast Candida boidinii was found to be required for methanol-regulated gene expression. In this study, we performed functional characterization of CbHap3p, one of the Hap complex components in C. boidinii. Sequence alignment of Hap3 proteins revealed the presence of a unique extended C-terminal region, which is not present in Hap3p from Saccharomyces cerevisiae (ScHap3p), but is found in Hap3p proteins of methylotrophic yeasts. Deletion of the C-terminal region of CbHap3p (Δ256–292 or Δ107–237) diminished activation of methanol-regulated genes and abolished the ability to grow on methanol, but did not affect nuclear localization or DNA-binding ability. However, deletion of the N-terminal region of CbHap3p (Δ1–20) led to not only a growth defect on methanol and a decreased level of methanol-regulated gene expression, but also impaired nuclear localization and binding to methanol-regulated gene promoters. We also revealed that CbHap3p could complement the growth defect of the Schap3Δ strain on glycerol, although ScHap3p could not complement the growth defect of a Cbhap3Δ strain on methanol. We conclude that the unique C-terminal region of CbHap3p contributes to maximum activation of methanol-regulated genes, whilst the N-terminal region is required for nuclear localization and binding to DNA.
Rights: © Saori Oda, et al., 2016. The definitive peer reviewed, edited version of this article is published in Microbiology, volume 162, issue 5, 2016, https://doi.org/10.1099/mic.0.000275.
This manuscript is under the Creative Commons Attribution 4.0 International license.
URI: http://hdl.handle.net/2433/266865
DOI(Published Version): 10.1099/mic.0.000275
PubMed ID: 26963751
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