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DCフィールド | 値 | 言語 |
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dc.contributor.author | Shiraishi, Kosuke | en |
dc.contributor.author | Hioki, Takahiro | en |
dc.contributor.author | Habata, Akari | en |
dc.contributor.author | Yurimoto, Hiroya | en |
dc.contributor.author | Sakai, Yasuyoshi | en |
dc.contributor.alternative | 白石, 晃將 | ja |
dc.contributor.alternative | 日置, 貴大 | ja |
dc.contributor.alternative | 幅田, 亜香莉 | ja |
dc.contributor.alternative | 由里本, 博也 | ja |
dc.contributor.alternative | 阪井, 康能 | ja |
dc.date.accessioned | 2021-12-28T07:07:24Z | - |
dc.date.available | 2021-12-28T07:07:24Z | - |
dc.date.issued | 2018-01 | - |
dc.identifier.uri | http://hdl.handle.net/2433/266866 | - |
dc.description.abstract | The yeast high-osmolarity glycerol (HOG) pathway plays a central role in stress responses. It is activated by various stresses, including hyperosmotic stress, oxidative stress, high-temperature stress and exposure to arsenite. Hog1, the crucial MAP kinase of the pathway, localizes to the nucleus in response to high osmotic concentrations, i.e. high osmolarity; but, otherwise, little is known about its intracellular dynamics and regulation. By using the methylotrophic yeast Candida boidinii, we found that CbHog1-Venus formed intracellular dot structures after high-temperature stress in a reversible manner. Microscopic observation revealed that CbHog1-mCherry colocalized with CbPab1-Venus, a marker protein of stress granules. Hog1 homologs in Pichia pastoris and Schizosaccharomyces pombe also exhibited similar dot formation under high-temperature stress, whereas Saccharomyces cerevisiae Hog1 (ScHog1)-GFP did not. Analysis of CbHog1-Venus in C. boidinii revealed that a β-sheet structure in the N-terminal region was necessary and sufficient for its localization to stress granules. Physiological studies revealed that sequestration of activated Hog1 proteins in stress granules was responsible for downregulation of Hog1 activity under high-temperature stress. | en |
dc.language.iso | eng | - |
dc.publisher | Company of Biologists Ltd | en |
dc.rights | © 2018. Published by The Company of Biologists Ltd | en |
dc.subject | High-temperature stress | en |
dc.subject | Hog1 | en |
dc.subject | Stress granule | en |
dc.subject | Yeast | en |
dc.title | Yeast Hog1 proteins are sequestered in stress granules during high-temperature stress | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Journal of Cell Science | en |
dc.identifier.volume | 131 | - |
dc.identifier.issue | 1 | - |
dc.relation.doi | 10.1242/jcs.209114 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | jcs.209114 | - |
dc.identifier.pmid | 29183915 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2019-01-09 | - |
datacite.awardNumber | 16J10395 | - |
datacite.awardNumber | 16K14883 | - |
datacite.awardNumber | 16K15089 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16J10395/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14883/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K15089/ | - |
dc.identifier.pissn | 0021-9533 | - |
dc.identifier.eissn | 1477-9137 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 植物葉上で増殖するメタノール酵母におけるストレス顆粒形成とその生理的意義の解明 | ja |
jpcoar.awardTitle | 過酷な環境変動とストレスに曝される葉面C1酵母:生存戦略の分子機構 | ja |
jpcoar.awardTitle | メタノール酵母の高レベル転写能を利用するメタノールからの機能性RNA生産 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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